Plug-in electric vehicles (PEVs) present environmental and energy security advantages versus conventional gasoline vehicles. In the near future, the number of plug-in electric vehicles will likely grow significantly in the world. Despite the aforementioned advantages, the connection of PEV to the power grid poses a series of new challenges for electric utilities. This paper proposes a comprehensive approach for evaluating the impact of different levels of PEV penetration on distribution network investment and incremental energy losses. The proposed approach is based on the use of a large-scale distribution planning model which is used to analyze two real distribution areas. Obtained results show that depending on the charging strategies, investment costs can increase up to 15% of total actual distribution network investment costs, and energy losses can increase up to 40% in off-peak hours for a scenario with 60% of total vehicles being PEV.Index Terms-Distribution investment, distribution network planning, electricity distribution, network energy losses, plug-in electric vehicles.