Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by positive selection with antibodies against the low-affinity nerve growth factor receptor (LNGFR or CD271), yielding a selective cell universe with higher proliferation and differentiation potential. This paper addresses the need for determining the quantity of ADSCs positive for the CD271 receptor and its correlation with donor's age. Mononuclear cells were harvested from the lower backs of 35 female donors and purified using magnetic beads. Multipotency capacity was tested by the expression of stemness genes and through differentiation into preosteoblasts and adipocytes. A significant statistical difference was found in CD271+ concentrations between defined age intervals. The highest yield was found within women on the 30–40-year-old age range. CD271+ ADSCs from all age groups showed differentiation capabilities as well as expression of typical multipotent stem cell genes. Our data suggest that the amount of CD271+ cells correlates inversely with age. However, the ability to obtain these cells was maintained through all age ranges with a yield higher than what has been reported from bone marrow. Our findings propose CD271+ ADSCs as the primary choice for tissue regeneration and autologous stem cell therapies in older subjects.

BACKGROUND In situ product recovery (ISPR), or extractive fermentation, is a strategy for the primary recovery of metabolites. A micellar aqueous two‐phase system (ATPS) composed of a surfactant‐rich phase was coupled to batch and continuous fermentations for the production and in situ recovery of the red alkaloid prodigiosin from Serratia marcescens. A cloud point ATPS was formed at 24 °C using triton X‐114 and used as a production/extraction system for this metabolite, which shows therapeutic properties as antibiotic, anti‐proliferative and anti‐malarial activities. RESULTS The integration of the micellar ATPS in batch fermentation resulted in the production of 342 mg prodigiosin L−1 with a recovery of 81% in the extractive phase. When a continuous fermentation was performed, based on a cloud point system, 1.2‐fold prodigiosin content was obtained compared with batch mode and a recovery of 88% of the metabolite. This continuous operation resulted in 0.43 g of purified prodigiosin from a 1.5 L bioreactor with a concentration of 872 mg prodigiosin L−1 of extractive phase and a purity of 82%, demonstrating particular selectivity of the system for prodigiosin as a primary recovery method. This is the first report on the continuous production of this generally recognized secondary metabolite. CONCLUSION The experimental approach proposed proved that an ISPR system could lead to the establishment of a more efficient process for continuous large‐scale prodigiosin production. The proposal of a primary recovery strategy with only one bioprocess step enhances this metabolite production by lowering the inhibiting properties of prodigiosin for the microorganism. © 2016 Society of Chemical Industry

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)