Three-dimensional (3D) printing of core/shell filaments allows direct fabrication of channel structures with a stable shell that is cross-linked at the interface with a liquid core. The latter is removed post-printing, leaving behind a hollow tube. Integrating an additive manufacturing technique (like the one described here with tailor-made [bio]inks, which structurally and biochemically mimic the native extracellular matrix [ECM]) is an important step towards advanced tissue engineering. However, precise fabrication of well-defined structures requires tailored fabrication strategies optimized for the material in use. Therefore, it is sensible to begin with a setup that is customizable, simple-to-use, and compatible with a broad spectrum of materials and applications. This work presents an easy-to-manufacture core/shell nozzle with luer-compatibility to explore core/shell printing of woodpile structures, tested with a well-defined, alginate-based scaffold material formulation. Video Link The video component of this article can be found at https://www.jove.com/video/59951/ Additive manufacturing (AM, such as 3D bioprinting) is increasingly involved in the fabrication of 3D constructs using biological or biocompatible materials to create scaffolds suitable for TE. Several AM approaches are being used and developed in parallel (e.g., ink jet-and microextrusionbased methods, different types of lithographic techniques) to produce scaffolds that mimic native tissues in their architecture, biochemistry, and functionality. The individual techniques exhibit certain advantages and disadvantages 28 , which is why various modifications being explored

Bioprinting has gained significant traction in recent years due to it's implications for medicine and research with a growing spectrum of potential applications. The focus of this work lies on developing an open-source piston driven syringe extruder with thermo-regulation, that is compatible with various CNC systems but also provides broad control and functionality. The manuscript describes the construction and evaluation of the extruder, as well as extrusion parameters and tested fabrication capabilities.

No abstract

Three-dimensional (3D) printing of core/shell filaments allows direct fabrication of channel structures with a stable shell that is cross-linked at the interface with a liquid core. The latter is removed post-printing, leaving behind a hollow tube. Integrating an additive manufacturing technique (like the one described here with tailor-made [bio]inks, which structurally and biochemically mimic the native extracellular matrix [ECM]) is an important step towards advanced tissue engineering. However, precise fabrication of well-defined structures requires tailored fabrication strategies optimized for the material in use. Therefore, it is sensible to begin with a set-up that is customizable, simple-to-use, and compatible with a broad spectrum of materials and applications. This work presents an easy-to-manufacture core/shell nozzle with luer-compatibility to explore core/shell printing of woodpile structures, tested with a well-defined, alginate-based scaffold material formulation.

No abstract