An algorithm for automatic digitization of pluviograph strip charts is presented. The rainfall signal is incrementally extracted from the scanned image of a strip chart by combining the moving average method and the curve edge following method. The mechanical properties of float-based rain gauge were used as constraints in the algorithm design. The algorithm was tested on 58 strip chart images. The comparison between the data derived from the algorithm and the data from the Slovenian Environment Agency shows that the algorithm produces an accurate rainfall time series except for the charts that contain ink smudges. Thus, the algorithm is well suited as a main component of an interactive system that would enable visual inspection of the detected rainfall curve and its possible adjustment.
Cell counting in microscopic images is one of the fundamental analysis tools in life sciences, but is usually tedious, time consuming and prone to human error. Several programs for automatic cell counting have been developed so far, but most of them demand additional training or data input from the user. Most of them do not allow the users to online monitor the counting results, either. Therefore, we designed two straightforward, simple-to-use cell-counting programs that also allow users to correct the detection results. In this paper, we present the Cellcounter and Learn123 programs for automatic and semiautomatic counting of objects in fluorescent microscopic images (cells or cell nuclei) with a user-friendly interface. Although Cellcounter is based on predefined and fine-tuned set of filters optimized on sets of chosen experiments, Learn123 uses an evolutionary algorithm to determine the adapt filter parameters based on a learning set of images. Cellcounter also includes an extension for analysis of overlaying images. The efficiency of both programs was assessed on images of cells stained with different fluorescent dyes by comparing automatically obtained results with results that were manually annotated by an expert. With both programs, the correlation between automatic and manual counting was very high (R(2) < 0.9), although Cellcounter had some difficulties processing images with no cells or weakly stained cells, where sometimes the background noise was recognized as an object of interest. Nevertheless, the differences between manual and automatic counting were small compared to variations between experimental repeats. Both programs significantly reduced the time required to process the acquired images from hours to minutes. The programs enable consistent, robust, fast and accurate detection of fluorescent objects and can therefore be applied to a range of different applications in different fields of life sciences where fluorescent labelling is used for quantification of various phenomena. Moreover, Cellcounter overlay extension also enables fast analysis of related images that would otherwise require image merging for accurate analysis, whereas Learn123's evolutionary algorithm can adapt counting parameters to specific sets of images of different experimental settings.
-The concept of the augmented coaching ecosystem for non-obtrusive adaptive personalized elderly care is proposed on the basis of the integration of new and available ICT approaches. They include multimodal user interface (MMUI), augmented reality (AR), machine learning (ML), Internet of Things (IoT), and machine-tomachine (M2M) interactions. The ecosystem is based on the Cloud-Fog-Dew computing paradigm services, providing a full symbiosis by integrating the whole range from low level sensors up to high level services using integration efficiency inherent in synergistic use of applied technologies. Inside of this ecosystem, all of them are encapsulated in the following network layers: Dew, Fog, and Cloud computing layer. Instead of the "spaghetti connections", "mosaic of buttons", "puzzles of output data", etc., the proposed ecosystem provides the strict division in the following dataflow channels: consumer interaction channel, machine interaction channel, and caregiver interaction channel. This concept allows to decrease the physical, cognitive, and mental load on elderly care stakeholders by decreasing the secondary human-to-human (H2H), human-to-machine (H2M), and machine-to-human (M2H) interactions in favor of M2M interactions and distributed Dew Computing services environment. It allows to apply this non-obtrusive augmented reality ecosystem for effective personalized elderly care to preserve their physical, cognitive, mental and social well-being.
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