We extend the concept of optical flow with spatiotemporal regularisation to a dynamic non-Euclidean setting. Optical flow is traditionally computed from a sequence of flat images. The purpose of this paper is to introduce variational motion estimation for images that are defined on an evolving surface. Volumetric microscopy images depicting a live zebrafish embryo serve as both biological motivation and test data.

We extend the concept of optical flow to a dynamic non-Euclidean setting. Optical flow is traditionally computed from a sequence of flat images. It is the purpose of this paper to introduce variational motion estimation for images that are defined on an evolving surface. Volumetric microscopy images depicting a live zebrafish embryo serve as both biological motivation and test data.

We propose a variational regularisation approach for the problem of template-based image reconstruction from indirect, noisy measurements as given, for instance, in X-ray computed tomography. An image is reconstructed from such measurements by deforming a given template image. The image registration is directly incorporated into the variational regularisation approach in the form of a partial differential equation that models the registration as either mass-or intensity-preserving transport from the template to the unknown reconstruction. We provide theoretical results for the proposed variational regularisation for both cases. In particular, we prove existence of a minimiser, stability with respect to the data, and convergence for vanishing noise when either of the abovementioned equations is imposed and more general distance functions are used. Numerically, we solve the problem by extending existing Lagrangian methods and propose a multilevel approach that is applicable whenever a suitable downsampling procedure for the operator and the measured data can be provided. Finally, we demonstrate the performance of our method for template-based image reconstruction from highly undersampled and noisy Radon transform data. We compare results for massand intensity-preserving image registration, various regularisation functionals, and different distance functions. Our results show that very reasonable reconstructions can be obtained when only few measurements are available and demonstrate that the use of a normalised cross correlation-based distance is advantageous when the image intensities between the template and the unknown image differ substantially.

In this work we consider optical flow on evolving Riemannian 2-manifolds which can be parametrised from the 2-sphere. Our main motivation is to estimate cell motion in time-lapse volumetric microscopy images depicting fluorescently labelled cells of a live zebrafish embryo. We exploit the fact that the recorded cells float on the surface of the embryo and allow for the extraction of an image sequence together with a sphere-like surface. We solve the resulting variational problem by means of a Galerkin method based on vector spherical harmonics and present numerical results computed from the aforementioned microscopy data.

We propose a number of variational regularisation methods for the estimation and decomposition of motion fields on the 2-sphere. While motion estimation is based on the optical flow equation, the presented decomposition models are motivated by recent trends in image analysis. In particular we treat u + v decomposition as well as hierarchical decomposition. Helmholtz decomposition of motion fields is obtained as a natural by-product of the chosen numerical method based on vector spherical harmonics. All models are tested on time-lapse microscopy data depicting fluorescently labelled endodermal cells of a zebrafish embryo.