Due to growing production, carbon nanotubes (CNT) may soon be found in a broad range of products and thus in the environment. In this work, an algal growth test was developed to determine effects of pristine and oxidized CNT on the green algae Chlorella vulgaris and Pseudokirchneriella subcapitata. CNT suspensions were prepared in algal test medium and characterized taking into account the suspension age, the reduced light transmittance of nanoparticle suspensions defined as shading of CNT and quantified by UV/vis spectroscopy, and the agglomeration of the CNT and of the algal cells. Growth inhibition and photosynthetic activity were investigated as end points. Growth of C. vulgaris was inhibited with effect concentrations of 50% (EC(50)) values of 1.8 mg CNT/L and of 24 mg CNT/L in well dispersed and in agglomerated suspensions, respectively, and 20 mg CNT/L and 36 mg CNT/L for P. subcapitata, respectively. However, the photosynthetic activity was not affected. Growth inhibition was highly correlated with the shading of CNT and the agglomeration of algal cells. This suggests that the reduced algal growth might be caused mainly by indirect effects, i.e. by reduced availability of light and different growth conditions caused by the locally elevated algal concentration inside of CNT agglomerates.

This study investigated the toxicity of double walled carbon nanotubes (DWCNTs) to three aquatic organisms, namely,Pseudokirchneriella subcapitata,Daphnia pulex, andPoecilia reticulataunder the influence of exposure media properties specifically the ionic strength and organic matter represented by humic acid. Results indicated that ionic strength enhanced DWCNTs agglomeration whilst humic acid stabilized the CNTs and in turn inhibited the formation of aggregates. LC50s forD. pulexwere higher at 2.81 and 4.45 mg/L for pristine and oxidised DWCNTs, respectively; however,P. reticulatahad lower values of 113.64 mg/L and 214.0 mg/L for the same CNTs correspondingly.P. subcapitatahad EC50s of 17.95 mg/L and 10.93 mg/L for the pristine and oxidised DWCNTs, respectively. In the presence of humic acid high DWCNTs acute toxicity towardsD. pulexandP. reticulatawas observed but ionic strength led to opposite effect irrespective of DWCNTs form. Both humic acid and ionic strength shielded theP. subcapitatafrom toxic effects of DWCNTs. Overall, our findings suggest that the toxicity of DWCNTs in the aquatic systems (i) will be dependent on media properties and (ii) is likely to proceed at different rates to organisms at different trophic levels.

Silver impregnated carbon nanotubes and cyclodextrin polymers were synthesised by first functionalising carbon nanotubes in a mixture of nitric and sulphuric acid before impregnating them with silver nanoparticles. The silver impregnated functionalised carbon nanotubes were then polymerised with β cyclodextrin using hexamethylene diisocyanate as the linker. The polymers were characterised using various techniques. The polymers were then tested for their ability to destroy bacteria in water and were found to reduce bacterial cell counts in water spiked with E. coli (ATCC 25925) to as low as zero cfu/mℓ. Furthermore, the polymers could absorb 58% of para-nitrophenol from water spiked with this organic compound, which is a known pollutant in water.

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