An unmet clinical need in solid tumor cancers is the ability to harness the intrinsic spatial information in primary tumors that can be exploited to optimize prognostics, diagnostics and therapeutic strategies for precision medicine. Here, we develop a transformational spatial analytics computational and systems biology platform (SpAn) that predicts clinical outcomes and captures emergent spatial biology that can potentially inform therapeutic strategies. We apply SpAn to primary tumor tissue samples from a cohort of 432 chemo-naïve colorectal cancer (CRC) patients iteratively labeled with a highly multiplexed (hyperplexed) panel of 55 fluorescently tagged antibodies. We show that SpAn predicts the 5-year risk of CRC recurrence with a mean AUROC of 88.5% (SE of 0.1%), significantly better than current state-of-the-art methods. Additionally, SpAn infers the emergent network biology of tumor microenvironment spatial domains revealing a spatially-mediated role of CRC consensus molecular subtype features with the potential to inform precision medicine.

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Segmenting a broad class of histological structures in transmitted light and/or fluorescence-based images is a prerequisite for determining the pathological basis of cancer, elucidating spatial interactions between histological structures in tumor microenvironments (e.g. tumor infiltrating lymphocytes), facilitating precision medicine studies with deep molecular profiling, and providing an exploratory tool for pathologists. Our paper focuses on segmenting histological structures in hematoxylin and eosin (H&E) stained images of breast tissues, e.g. invasive carcinoma, carcinoma in situ, atypical and normal ducts, adipose tissue, lymphocytes. We propose two graph-theoretic segmentation methods based on local spatial color and nuclei neighborhood statistics. For benchmarking, we curated a dataset of 232 high power field breast tissue images together with expertly annotated ground truth. To accurately model the preference for histological structures (ducts, vessels, tumor nets, adipose etc.) over the remaining connective tissue and non-tissue areas in ground truth annotations, we propose a new region-based score for evaluating segmentation algorithms. We demonstrate the improvement of our proposed methods over the state-of-the-art algorithms in both region and boundary based performance measures.

Nowadays, online transactions are becoming more and more popular in modern society. As a result, Phishing is an attempt by an individual or a group of people to steal personal information such as password, banking account and credit card information, etc. Most of these phishing web pages look similar to the real web pages in terms of website interface and uniform resource locator (URL) address. Many techniques have been proposed to detect phishing websites, such as Blacklist-based technique, Heuristic-based technique, etc. However, the numbers of victims have been increasing due to inefficient protection technique. Neural networks and fuzzy systems can be combined to join its advantages and to cure its individual illness. This paper proposed a new neuro-fuzzy model without using rule sets for phishing detection. Specifically, the proposed technique calculates the value of heuristics from membership functions. Then, the weights are generated by a neural network. The proposed technique is evaluated with the datasets of 11,660 phishing sites and 10,000 legitimate sites. The results show that the proposed technique can detect over 99% phishing sites. 