Wounds infection is one of the most major problems in surgical " department related " to surgery, burns unit and orthopedic unit..The present study was to isolate and identify the bacteria causing wound infection and to determine the most effective antibiotic resistance to bacteria.This study was conducted in Kirkuk city from November 2018 to March 2019 , the total number of samples 27, isolated from Kirkuk General Hospital and Azady Teaching Hospital .In this study,13 males and 14 females were taken. Samples were taken from various hospital units(surgical department, burns and orthopedic units). We cultured the samples on the MacConkey Agar and Blood Agar. Then the antibiotic sensitivity was determined against the bacterial isolated from wound infections.A total of 27 patients were studied of which 18 were completely followed up for wound infection. Females were predominant (72%). Of the 18 patients, (27.7%) were identified as E. coli,(22.2 %) Klebsiella,(16.6%) Citrobacter,(11.1%) Proteus, (11.1%) Staphylococcus aureus and (11.1%) Staphylococcus epidermides. the most bacteria that appeared in wound infection is E. coli, and the infection in females more than in males, the most effective antibiotics were Ciprofloxacin, Amikacin and Rifampicin 55.5%