Lecturer usually uses media in order to help students' understanding about organic chemistry. In this research, the aim is to find out the ability and perception of students in learning by using media in the form of online and offline. The offline media we used was namely Spartan Student V6, and ChemAxon Reactor software, while the online media used is Reaction Predictor. Participants from this study were 16 students from one of the state universities in Papua. This research is a descriptive research by using the percentage of data and from questionnaire results. It shows the ability of students' in using media.. Based on the results of this study, it was found that the ability of students in using mediathrough the learning of organic synthesis was still low, around 10-40%. The media that highest used is Reaction Predictor onlinemedia and the lowest used is Spartan Student V6 offline media. It was also known that the results of students' responses for using media in learning synthetic organic chemistry are positive.