In order to study the real-time control research of laboratory information system based on nonlinear programming, by analyzing the role played by the laboratory information management system (LIMS) in quality management, and the relationship with quality control, put forward the functions that LIMS should set up and possess in the management of controlled documents, processing of inspection data, etc., an accurate and reliable method to ensure the test results. It has been verified that the system can meet the performance requirements of 350 virtual concurrent users, its memory usage and CPU usage are within acceptable limits, the response time of important operations of each module of the system is within 3 s. The average memory consumption obtained from the test is 2.02 GB, and the average utilization rate of CPU is 66%. The two indicators basically meet the performance requirements of the system. The system can meet the performance requirements of the number of virtual concurrent users 350, both its memory occupancy and CPU utilization rate are in the acceptable range. It not only improves the accuracy of the sample processing results, but also reduces the labor intensity of the technicians, so that the laboratory expands the analysis function from the simple reporting function.