Formula L1α-Pinene 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene 6654 136.23 C10H16 L2 Sabinene 4-methylidene-1-propan-2ylbicyclo[3.1.0]hexane 18818 136.23 C10H16 L3 β-pinene 6,6-dimethyl-2methylidenebicyclo[3.1.1]heptane 14896 136.23 C10H16 L4 Myrcene 7-methyl-3-methylideneocta-1,6-diene 31253 136.23 C10H16 L5 p-Cymene 1-methyl-4-propan-2-ylbenzene 7463 134.22 C10H14 L6 Limonene 1-methyl-4-prop-1-en-2-ylcyclohexene 22311 136.23 C10H16 L7 (Z)-β-ocimene (3Z)-3,7-dimethylocta-1,3,6-triene 5320250 136.23 C10H16 L8 (E)-β-ocimene (3E)-3,7-dimethylocta-1,3,6-triene 5281553 136.23 C10H16 L9 Nonanal nonanal 31289 142.24 C9H18O L10 Linalool 3,7-dimethylocta-1,6-dien-3-ol 6549 154.25 C10H18O L11 Terpinen-4-ol 4-methyl-1-propan-2-ylcyclohex-3-en-1-ol 11230 154.25 C10H18O L12 methyl-Salicylate methyl 2-hydroxybenzoate 4133 152.15 C8H8O3 L13 Decanol decan-1-ol 8174 158.28 C10H22O

Background: In herbal medicine, Centaurea are used in the treatment of many diseases such as dizziness, headaches, reduce inflammatory pain and liver disease. The roots of Centaurea acaulis and Centaurea pullata have not yet been studied for biological properties. Objective: The aim of this research was to evaluate the chemical composition and the anti-inflammatory, antioxidant and neuroprotective properties of hexane extracts of Centaurea acaulis and Centaurea pullata roots, and their major component, aplotaxene. Method: The hexane extract was prepared by the maceration process and identified by GC and GC-MS. Aplotaxene was isolated by flash chromatography. The Antioxidant activity was assessed using DPPH, the β-carotene bleaching and FRAP methods. Anti-inflammatory effect was assessed by egg albumin denaturation assay and the neuroprotective activity was assessed against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Results: The chemical composition of hexane extract of Centaurea pullata was mainly represented by non-terpenic compounds such as Aplotaxene (80.3%), while, hexane extract of Centaurea acaulis was characterized by high levels of Aplotaxene (56.9%), 9-oxabicyclo(6,1,0)nonane (9.2%), Caryophyllene oxide (8.3%) and Isocaryophyllene (6.0%). The hexane extracts of the two Centaurea showed very good antioxidant activities with all three methods. Aplotaxene has shown excellent antioxidant activity compared to BHT and ascorbic acid. Centaurea acaulis hexane extract showed very high anti-inflammatory activity with an IC50 of 0.76 mg/L in the egg albumin denaturation test compared to diclofenac (IC50 of 1.01 mg/L). The extract of Centaurea pullata and Aplotaxene showed an interesting anti-inflammatory activity with IC50s of 1.72 and 1.36 mg/L, but which remains lower than that of diclofenac sodium. The neuroprotective activity of Centurea pullata and Centaurea acaulis extracts, and Aplotaxene did not show inhibition against AChE, whereas they inhibited BChE with IC50 values of 92.3, 583, and 81.5 mg/L, respectively. Conclusion: Further analysis is still needed to further demonstrate the biological efficacy of Centaurea acaulis and Centaurea pullata extracts and Aplotaxene.

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