Background: Cancer is still a major health problem worldwide, despite the big development in treatment and diagnostic methods. For this reason, this study aims to find new drugs with low cost and fewer side effects on cancer cells continue. Medical plants represent a suitable candidate for discovering new materials for cancer treatment. Methods: Ten plants usually used in traditional medicine were used to evaluate their effectiveness as anti-cancer. These plants were divided into two mixtures according to traditional usage, named mixtures A and B. The anticancer effect against HepG2 was determined by MTT assay. The IC50 was also calculated. Result: Mixture A showed cytotoxicity reached 66.98%, while mixture B showed 63.66% by using 400 µg/ml at 4 8h. The IC50 for mixture A was 53 µg/ml, while for mixture B was 44.29 g/ml. All these results compare to normal cell line HnFd cells. In conclusion, mixing medical plants can effectively increase the anticancer activity of the synergistic effect between the phytochemicals. Future studies will be done to scan these effects.