Aim. Research objective is to establish the possibility of modifying the astaxanthin (a carotenoid from a xanthophyll group) radiation-induced cytogenetic effects in human peripheral blood lymphocytes (PBLs) in vitro. Methods. The cultivation of PBLs from four conventionally healthy volunteers, the preparation and analysis of uniformly stained slides of metaphase chromosomes. Astaxanthin in final concentrations of 2, 10 and 20 µg/ml was added into the culture of PBL prior to the incubation before irradiation with γ-quanta in a dose of 1 Gy. Results. Astaxanthin did not affect the level and spectrum of chromosome damage in non-irradiated PBLs both in individual persons, and along the group on average (P>0.05), indicating a lack of mutagenic activity. The effect of astaxanthin at a concentration of 20 μg/ml on irradiated PBLs resulted in a significant reduction of radioinduced cytogenetic effect in all donors. Medium-group level of chromosome aberrations decreased almost 3 times and was characterized by statistically significant (P<0.001) decrease in frequency of chromosomal type aberrations due to the classical unstable cytogenetic markers of radiation effect, dicentrics and ring chromosomes. Conclusions. Astaxanthin at a concentration of 20 µg/ml was found to reduce the mutagenic effect of ionizing radiation, thus suggesting its powerful radioprotective potential. Keywords: astaxanthin, culture of human peripheral blood lymphocytes, radiation mutagenesis, chromosome aberrations, radioprotective effect.

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Одним із проявів універсального феномену bystander response як відповіді нормальних клітин (свідків) на генотоксичний стрес, індукований клітинами-мішенями, є нещодавно відкритий прямий і зворотний пухлино-індукований ефект свідка (tumor-induced bystander effect (TIBE))-результат взаємодії між здоровими та злоякісними клітинами [1, 2]. Здатність до індукції ТІВЕ встановлено, зокрема, для клітин меланоми та аденокарциноми легенів за умов їх сумісного культивування з нормальними клітинами з різних тканин люди

The chapter is devoted to study the effects of astaxanthin on the frequency of chromosomal aberrations and the level of DNA damages in human peripheral blood lymphocytes under ionizing radiation exposure in vitro. To achieve the purpose of the research, a combination of classical cytogenetic methods (G 0-and G 2-radiation sensitivity assays) and method of single-cell electrophoresis (comet assay) was used. The specificity of the modifying effect of astaxanthin on radiation-induced genomic injuries depending on the stage of the cell cycle had been determined. Significant weakening of the negative effect of ionizing radiation on the G 0 stage and the absence of a radioprotective effect on the S and G 2 stages of the cell cycle may be associated with activation by astaxanthin of apoptosis in irradiated cells with a critically high level of the genome damages. The research results not only testify about strong radioprotective effect of astaxanthin but also demonstrate the feasibility of the parallel use of cytogenetic and molecular genetic methods to assess the impact as mutagens as well as factors that modify the effect of mutagens on genome stability.

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