RNFL and GCC thickness was significantly decreased both in MS+ON and MS-ON groups compared to controls. No difference in the annual change of RNFL thickness and GCC parameters between patients with ON and patients who did not have any visual impairment in the anamnesis was found. Changes of GCC were detected only in patients with secondary progressive MS. The study of GCC and RNFL thickness can be used to describe and characterize the level of axonal damage in MS.