Hyaena is one of the twenty seven mammals species protected by National Park and Wildlife conservation ACT-1973 of Nepal. Study of hyaena is lacking in Nepal so less information is available about this species in country. A single den was recorded in Kalapani community forest of Deukhuri Valley in Dang District located at the elevation of 282m above sea level. Signs, camera trap images and video confirm the den of striped hyaena which is first confirmed record of Hyaena den in Nepal. [3][4][5] where water is available within 10 km [6,7]. They appear to avoid open desert and dense thickets and forests [6][7][8] and have only been found below 3,300 m [6,7,9]. Striped hyaena generally favors and consistently revisits larger caves for resting [6,7,10,11]. The striped hyaena is monogamous, with the male helping the female to establish a den, raise young and feed her when cubs are born. Striped hyena cubs are reared in dens and intense digging behaviour in the females announces parturition [6]. Dens may be holes dug by the mother, holes formed and abandoned by other species [9,12] or deep, natural, and sometimes complex, caves [8,11,13]. Striped hyaena is one of the protected mammals by National Park and Wildlife conservation ACT, 1973AD. Feeling the important of this species and relatively less information available striped hyaena was kept as a protected mammal of Nepal. Wildlife act-1973 [2,14-18] are past evidence which shows the presence records of hyaena in different period in Nepal. This all provides only the presence evidence of Hyaena in Nepal. Research on this species is limited so there is little information about this species and no any records of den are available in country before. This is the first confirmed documentation of Hyaena den in Nepal (Figure 1). First Confirmed Record of Striped Hyaena (Hyaena hyaena) Den in NepalInformation about the den and its location was firstly known through the local forest users and forest guard of the Kalapani community forest of Deukhuri Valley in late 2012. Signs around the den were recorded and the confirmation of signs was done in 2013. When the signs were confirmed to be of striped hyaena further confirmation was done through photos and videos obtained from camera trapping. Hyaena is also using this den for breeding. Camera trapping done in the den site for four years (2014 to2017) shows that hyaena are breeding in this den. Two cubs along with adult male and female were recorded in 2015 and recently in July and august 2017 two cubs are seen in den.Camera trap programmed in day and night activation mode was placed at the base of a Dalbergia sissoo and Acacia catechu focusing on the cave entrance (Figure 2). After the assurance through camera trap images and videos, morphometric measurements of the dimensions of the den followed by ocular survey of the surrounding habitat was carried out. The periphery of the den was examined to look for the presence of further entrances and also for the possible carcasses of hyaena prey. We recorded bones and skull of d...