Water purification and reduction of water borne diseases is a major public health goal. The study was aimed to utilize Moringa oleifera seeds extract for purification of ground water at Challawa industrial area Kumbotso Local Government Kano State, Nigeria. Ground water was collected from four different locations in the area. All water samples were subjected to bacteriological and physiological analysis using standard laboratory methods. Extract from the seeds of Moringa oleifera was used in the process of purifying the water as antibacterial and anticoagulant agent. The result showed that 5 bacterial isolates (Escherichia coli, Salmonella typhi, Proteus mirabilis, Enterobacter spp and Klebsiella pneumoneae) were recovered and all were susceptible to Moringa oleifera seeds extract. The most susceptible isolate is E. coli with aveage zone of inhibition of 17.82 mm while Klebsiella pneumoneae is the least susceptible with average zone of inhibition of 13.00 mm. The turbidity assessment showed that at a concentration of 1000mg/L the Moringa seed suspension can reduce the turbidity of ground water from 4NTU to 1NTU after exposing for 90 minutes. Moringa, therefore, could be regarded as one of the remedy to reduce the incidence of water borne disease leading to high incidence of death especially in the developing world.