The confinement by COVID-19 has meant a re-reading of housing for Spanish households, resulting in the only available and safe space to carry out daily activity. This complex phenomenon has generated a completely different way of inhabiting it, as well as of relating to domestic spaces. For this reason, the home perception and its characteristics must be evaluated, highlighting those perceived as deficiencies, or as preferences in such an unusual context as lockdown, where the experience was different depending on the dwelling characteristics, and the family in question. To deepen in this double perception home-dwelling, a mixed method was used, with two online forms. The first is a quantitative questionnaire, while the second asks the participants for photographs and narratives about such images. More than 1800 surveys and 785 qualitative responses were obtained. From both approaches, the joint discourse arose, allowing an exploratory analysis of the current situation of the Spanish residential park, and the resilience demonstrated in this period by both households and their usual dwellings. This study should facilitate the development of new proposals on housing in contexts similar to the COVID-19 pandemic.

No abstract

Pyruvate kinase deficiency (PKD) is a monogenic metabolic disease caused by mutations in the PKLR gene that leads to hemolytic anemia of variable symptomatology and that can be fatal during the neonatal period. PKD recessive inheritance trait and its curative treatment by allogeneic bone marrow transplantation provide an ideal scenario for developing gene therapy approaches. Here, we provide a preclinical gene therapy for PKD based on a lentiviral vector harboring the hPGK eukaryotic promoter that drives the expression of the PKLR cDNA. This therapeutic vector was used to transduce mouse PKD hematopoietic stem cells (HSCs) that were subsequently transplanted into myeloablated PKD mice. Ectopic RPK expression normalized the erythroid compartment correcting the hematological phenotype and reverting organ pathology. Metabolomic studies demonstrated functional correction of the glycolytic pathway in RBCs derived from genetically corrected PKD HSCs, with no metabolic disturbances in leukocytes. The analysis of the lentiviral insertion sites in the genome of transplanted hematopoietic cells demonstrated no evidence of genotoxicity in any of the transplanted animals. Overall, our results underscore the therapeutic potential of the hPGK-coRPK lentiviral vector and provide high expectations toward the gene therapy of PKD and other erythroid metabolic genetic disorders.