Aims: This study aimed to evaluate the influence of maternal factors on the total plate count, Staphylococci, Lactobacilli and Bifidobacteria populations in the milk of lactating Filipino women for the first 4 months of lactation period. Methods and Results: Milk samples (n = 136), 24 h diet recalls and responses from a qualitative food frequency questionnaire (FFQ) were collected from 34 healthy lactating women classified according to their BMI: underweight (n = 7), normal weight (n = 16) and overweight (n = 11). The FFQ was useful in assessing prebiotic and probiotic food items consumed by the participants. Microbial populations were enumerated using culture-plating method, and showed a nonsignificant decreasing trend suggesting their relative stability throughout the first 4 months of lactation. Carbohydrate and fat intakes were associated with TPC, and with both TPC and Staphylococci respectively (P ≤ 0Á05); and consumption of root crop is linked with Staphylococci, Lactobacilli and Bifidobacteria (P ≤ 0Á05). Interestingly, age was found to be a positive determinant for Bifidobacteria (P = 0Á00), whereas being normal-or overweight as negative determinants of Lactobacilli (P = 0Á017). Consumption of milk also seems to positively influence both Lactobacilli (P = 0Á00) and Bifidobacteria (P = 0Á05) counts. Conclusions: Certain populations of culturable micro-organisms were found to be associated with maternal diet, weight classification and age. Significance and Impact of the Study: This study offered new discoveries in the recently growing endeavor on the role of maternal factors in modulating certain microbial populations in human milk. Ultimately, the findings of this study could provide a basis in crafting lactation policies and guidelines that may help enhance the microbial quality of human milk through adjustments in maternal diet or weight during lactation.

Objective. Mothers are exposed to various functional food components, including probiotics, which have a direct effect on the quality of their breast milk. Probiotics enhance the microbial composition of breast milk, making it more beneficial for the infant’s gut health. This study aimed to explore the extent of knowledge and dietary practices on probiotics among low- to middle-income healthy Filipino mothers in an urban area.Methods. Data was gathered using three focus group discussions (FGD) participated in by low- to middle-income healthy Filipino mothers residing in Barangays West Rembo and Rizal in Makati City, Philippines. Prior to the FGD, preliminary interviews of selected participants and market surveys were done to determine the food items to be included in the FGD questionnaire.Results. A total of 19 mothers participated in the FGDs. The most common probiotic sources identified were yogurt, fermented milk product (Yakult), miso and tausi. The most consumed probiotic source was fermented milk product (94.7% of participants), at least once a month; followed by powdered milk (78.9%), consumed daily. The most commonly reported influence for consumption of these food items was advertisements from television (89.5%). About 53% of the participants related probiotics to better digestion and increased immunity as heard from advertisements and through counselling by physicians. When asked to elaborate on how probiotics influence digestion and immunity, almost all participants had difficulty explaining the benefits.Conclusion. Although majority of the participants consumed food sources of probiotics, they had limited knowledge on its other non-food sources and benefits. In addition, the consumed food source was confined to the food item that was most advertised on television. Thus, there is a need for health and nutrition experts to exert a stronger influence in the community by disseminating information regarding probiotics.