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Aims:To carry out phytochemical screening, acute toxicity test and to investigate the hypoglycemic potential of the ethanol extract and fractions of Globimetula braunii leaves.

The plant Adenodolichos paniculatus is a shrub of up to 4m high of Savanna (bush and jungle) from Guinea to Northern Nigeria and across to Sudan. The plant has been reported to be used traditionally in tooth ache, blennorrhea and in treatment of burns. In this work, the analgesic and anti-inflammatory activities of the methanolic leaf extract of Adenodolichos paniculatus (MAP) were studied. MAP (75, 150 and 300 mg/kg i.p) was evaluated for analgesic and anti-inflammatory activities using acetic acid induced writhing and Carrageenan induced paw edema in mice and rats respectively. The methanolic leaf extract exhibited significant (P < 0.05) and dose dependent analgesic and anti-inflammatory effects comparable to that of the reference drug Ketoprofen (20 mg/kg body weight i.p) which is a standard analgesic and anti-inflammatory drug. The preliminary phytochemical screening of the MAP revealed the presence of flavonoids, tannins, glycosides, anthraquinones and phenols. The intraperitoneal median lethal dose (LD 50 ) of MAP in mice was found to be 1113.3mg/kg and the oral LD 50 in rats was found to be greater than 5,000 mg/kg. body weight. The findings suggest that the MAP possesses analgesic and anti-inflammatory activities, and supports the ethno medical claims of the use of the plant in the management of painful and inflammatory conditions.

In present study acute oral toxicity value and the effect of the methanolic leaf extract of the Adenodolichos paniculatus (MAP) on function status of the liver were investigated in rats after two weeks of daily oral administration of graded doses ( 250, 500 and 1000 mg/kg body weight) of the extract. The preliminary phytochemical screening of the plant revealed the presence of flavonoids, tannins, steroids, carbohydrates, glycosides, alkaloids, anthraquinones and cardiac glycosides. The oral LD 50 of the extract in rats was found to be greater than 5000 mg/kg body weight suggest that the extract is relatively non-toxic and safe. Results obtained from the study showed that all values for biochemical parameters (ALT, ALT, TB and CB) for the control and experiment groups were statistically insignificant (P < 0.05) in all the graded doses used (250, 500, 1000 mg/kg body weight) except for AST which is statistically significant (P > 0.05) when compared with control group in all the graded doses used (250, 500, 1000 mg/kg body weight). The extract does not relatively show a dose dependent alteration in liver enzymes functions. It could be suggest that the methanolic leaf extract of Adenodolichos paniculatus had no adverse effects on the functional status of the liver at doses tested and for period of the study. Sub chronic and chronic toxicity study is required to ascertain this claim.