Mungbean Yellow Mosaic Virus (MYMV), transmitted through the white fly (Bemisia tabaci) in the persistent manner is one of the most pernicious diseases of Vigna species. Twenty-five genotypes of mungbean [Vigna radiata (L.) Wilczek] were sown in randomized block design with two replications during the summer season in 2015. The test entries were evaluated against the mungbean yellow mosaic virus (MYMV) under natural field conditions. Screening for MYMV resistance was done by planting infector rows along with the test entries. Percent Disease Incidence (PDI) was calculated. The differential response against MYMV observed and the results revealed that most of the genotypes studied were characterized as moderately susceptible to highly susceptible. In spite of the variable response to MYMV, IPM 02-03, KM 2241, PDM 139, Pusa 0672, HUM 16, ML 1464 and TARM-1 of the mungbean genotypes exhibited resistance during the summer, 2015. The present investigation suggests that these genotypes could possibly be utilized as donors to develop MYMV resistant lines MYMV resistance by introgressing in agriculturally important but MYMV susceptible genotypes. Consequently, in the near future, the improved varieties may surfeit the sustainable agriculture production in the biotic stress prone areas.