Cotton is one of the most important fibre crops in the world. An increase in ploidy level was observed in diploid cotton species namely Gossypium herbaceum in the experiment, through colchicine application. There were significant growth variations noticed during the induction of polyploidy in the cotton plants depending upon the concentration of colchicine, duration of the treatments and genotypes taken. An increase in the concentration of colchicine or the duration of the treatment had a retardation effect on seed viability in G. herbaceum. The hypocotyls of length between 4 and 8 mm were found to be most responsive to colchicine treatment. The root meristem of G. herbaceum, treated with 0.2 and 0.4% colchicine exhibited the most number of tetraploid cells during 16 h of treatment. The increase in the concentration of colchicine, along with an increase in duration of treatment led to the chromosomal abnormalities in the wild cotton species. Seed treatment for colchicine application, was the most efficient and reliable method when compared to Petri-plate application and cotton swabbing treatments for inducing polyploidy in diploid cotton.

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The prospective of Peroxidase Isozyme biochemical marker, have been investigation in the present study, ten pearl millet hybrids [Pennisetum glaucum (L.) R. Br.] Genotypes were used to examine the suitability of peroxidase enzymes for characterization of pearl millet genotypes. Band number 1 (Rm = 0.120) was present in three hybrids i.e. GHB-526, GHB-538 and GHB-719. Band number 2 (0.158) was also present in three hybrids i.e. GHB-538, GHB-577 and GHB-719. Band number 3 (Rm = 0.380) was present in only one hybrid i.e. GHB-719 while band number 4 (Rm = 0.429) was present in five hybrids GHB-538, GHB-719, GHB-732, GHB-235 and GHB-757. All the seven bands were present in only one hybrid GHB-719. 9 DAG stage was found to be more effective as compared to 3 DAG and 6 DAG by showing more number of bands. Among the 10 genotypes studied, two could be differentiated from each other Total 13 bands of peroxidase isozymes were observed at 3, 6 and 9 day after germination (DAG). A total of 15 alleles were generated by isozymes at different DAG (3, 6 and 9 days) with an average of 5 bands per day. Banding pattern at 6 DAG showed that band number1, 2 and 3 (Rm = 0.525, 0.573 and 0.600) were present in all the hybrids except for GHB-744, in band number 1. At 9 DAG seven bands of peroxidase isozymes were observed having Rm value of 0.120, 0.158, 0.380, 0.429, 0.430, 0.478, and 0.760. Key words : Genetic purity, Pearl millet hybrids, ADH, GOT How to cite this paper : Vaja, M.B., Rathod, P.J. and Mandavia, M.K. (2015). Genetic purity and diversity in isoenzyme Peroxidase of pearl millet hybrids [Pennisetum glaucum (L.) Br. R.]. Asian J. Bio. Sci., 10 (2) : 153-157.

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