I and II plasma concentrat ions in age-matched (M±ES: CA t t .29±0.38 and 11.95±0.94, BA 9 97±0.55 and 9.95±0.93) pts presenting with TS and on the IGF-1 generation by fibrobla sts in cu ltu re from 3 TS pts and t control. IG F-11 plasma concentratio n was normal and unresponsive to the treatment. IGF-1 plasma basal concentration was normal and increased significantly in both groups afte r 12 mo nths of treatm ent. No difference was fou nd between the two treatment regimens. Furthermore, we verified th e abi lity of the fibrob lasts from TS pts to synthetize in vitro the IGF•I in basal and GH, EE or GH + EE stimulated conditions. The results indicate that TS fibroblasts produce less IGF•I than fibroblasts from control; this finding can account for a reduced paracrine/autocrine action of IGF-1 in these pts, in spite of a normal IGF-1 plasrna concentration. Addition of in creasing concentrations of GH (0•1 00 ng/ml of medium) induced a dose-dependent increase of IGF-1 in th e co nditioned medium with a maximum at 50 ng/ml of GH. Addition of EE (at 0-500 pg /m l of medium) also result ed in an incre ased production of IGF-1 with a maximum already at 25 pg/ml of EE. The combination of increasing do ses of GH with 50 pg/ml of EE was additive only in the fibroblasts from one pt.

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