Aim of the research: to identify changes in the state of the prooxidant-antioxidant system in the organs of different plant varieties, depending on their level of resistance to disease. The subject of the research is the role of individual components of the prooxidant-antioxidant system in ensuring plant resistance to disease. Methodology. Quantitative determination of PAS status was performed on onion tissue samples taken from the following varieties: "Globus" (high-resistant variety), "Rainbow" (medium-resistant variety) and "Donetsk Golden" ( low-resistant variety). For biochemical analysis, tissues from the top of the leaf, the middle of the leaf, the scales of the onion-turnip, stem, flower, roots, and seeds were used. The concentration of superoxide anion radical, TBA-active products, superoxide dismutase activity, catalase, GSH-peroxidase, the concentration of ascorbic acid, glutathione, cytochrome oxidase activity were determined. The results of the research show that іn the tissues of photosynthetic vegetative organs of onions, there is an increase in both parts of the prooxidant-antioxidant system; in tissues that are not capable of photoproduction, there is an advantage of the antioxidant link in accordance with the increased resistance of the variety to disease. Initiation of germination processes enhances the activity of both parts of the prooxidant-antioxidant system and is highest in flower cells. Onion seed tissues, which are at rest, have the advantage of a prooxidant link and an increase in the concentration of low molecular weight antioxidants. Practical consequences. As a result of the correlation analysis of the studied indicators, the presence of a close relationship between the concentration of TBA-active products and the activity of cytochrome oxidase and superoxide dismutase, ascorbate with glutathione was established.
Aim of the research: to identify changes in the value of indicators of the state of the prooxidant-antioxidant system (PAS) in the tissues of edible parts of agricultural plants under the influence of temperature changes. Methodology: Quantitative determination of indicators of the state of PAS was performed on tissue samples of edible parts of the following plants: Solánum lycopérsicum L., Сucumis sativus L., Capsicum annuum L., Solanum melongena L., Solanum tuberosum L., Allium sativum L., Allium cepa L., Daucus carota L., Beta vulgaris L., Cucurbita pepo var. Giraumontia L. The concentration of superoxide anion radical (•O2-), TBA-active products, superoxide dismutase (SOD) activity, catalase, the concentration of ascorbic acid (AA), glutathione (GSH) were determined. The results of the research show that hypothermia activates both parts of the PAS, however, cooling is accompanied by more powerful both low molecular weight and enzymatic antioxidant (AO) protection. The research of AO can be divided according to the degree of reduction of the protective value in hypothermia in the following series: SOD, catalase, GSH, AA. The most resistant in terms of changes in PAS to hypothermia is Solanum tuberosum L., Allium sativum L., Beta vulgaris L.; the least resistant is Capsicum annuum L., and Solánum lycopérsicum L. The generative organs of plants are less resistant to hypothermia than the vegetative ones. Practical consequences. As a result of the conducted biochemical analysis, it is established which method of storage of plant products is more effective in terms of preservation of AO activity: cooling or freezing.
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