In entrepreneurial ecosystems (EEs), geographical and contextual factors play a big role in shaping the knowledge bases for digital innovation. While cities around the world compete to be perceived as successful “tech startup hubs”, proactive urban strategies are needed to create knowledge spillovers into EEs. This study explores the evolution of artificial intelligence (AI) knowledge practices in the EEs of Berlin and Sydney by using knowledge-spillover theory of entrepreneurship. The study utilizes a bibliometric analysis of secondary data in combination with exploratory stakeholder interviews conducted for both cities. Findings underline the critical role of experimental knowledge in driving the momentum of the EEs and the supporting role of policies imprinting knowledge practices. The paper shows how the dynamics of EEs can be explored empirically and raises awareness of the role of specialised and integrated policies in determining a city’s overall success in building EEs.
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