The aim of this study was to investigate variations in soil organic carbon (SOC) for two soil types and six common land uses in the New South Wales Murray Catchment and to explore the factors influencing those variations. Samples were collected from 100 sites on duplex soils (Ustalfs) of the Slopes region, and 100 sites on red-brown earths (Xeralfs) of the Plains region. Stocks of SOC (0–30 cm) across the study area ranged between 22.3 and 86.0 t ha–1, with means (± s.e.) of 42.0 ± 1.3 and 37.9 ± 0.8 t ha–1 for the Slopes and Plains regions, respectively. Higher SOC stocks were present in pasture-dominated land uses compared with mixed cropping in the Slopes region, with particularly high stocks found in pastures at positions on a slope of 7–10%. No significant differences in SOC stocks were identified between land-use groups (pastures or cropping) in the Plains region (<500-mm rainfall zone). Significant correlations were found between SOC and a range of climatic, topographical, and soil physico-chemical variables at both the catchment and sub-regional scale. Soil physico-chemical and topographical factors play an important role in explaining SOC variation and should be incorporated into models that aim to predict SOC sequestration across agricultural landscapes.

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