Gender inequality of women participation in agricultural production has become a worrisome discussion in the present dispensation. This is because women constitute large portion in agriculture business. The study therefore examines this issue, stressing on the extent of women’s participation in agriculture. The highest frequency of women that engage in farming all year round is 48 (30.0%), 44 (27.6%) respondents engage in farming activities half a year, 35 (21.8%) engage quarterly in a year. While cultural practices 36 (22.5%) form the highest causes of gender inequality and women’s participation in agricultural production in Warri South Local Government Areas of Delta State, using data collected through questionnaire interview from 160 subjects. The study reveals that gender inequality of women in agricultural participation has considerable impact on the production process.