Phycobiliproteins are a group of colored proteins commonly present in cyanobacteria and red algae possessing a spectrum of applications. They are extensively commercialized for fluorescent applications in clinical and immunological analysis. They are also used as a colorant, and their therapeutic value has also been categorically demonstrated. However, a comprehensive knowledge and technological base for augmenting their commercial utilities is lacking. Hence, this work is focused towards this objective by means of analyzing global patents and commercial activities with application oriented research. Strategic mining of patents was performed from global patent databases resulting in the identification of 297 patents on phycobiliproteins. The majority of the patents are from USA, Japan and Europe. Patents are grouped into fluorescent applications, general applications and production aspects of phycobiliproteins and the features of each group are discussed. Commercial and applied research activities are compared in parallel. It revealed that US patents are mostly related to fluorescent applications while Japanese are on the production, purification and application for therapeutic and diagnostic purposes. Fluorescent applications are well represented in research, patents and commercial sectors. Biomedical properties documented in research and patents are not ventured commercially. Several novel applications are reported only in patents. The paper further pinpoints the plethora of techniques used for cell breakage and for extraction and purification of phycobiliproteins. The analysis identifies the lacuna and suggests means for improvements in the application and production of phycobiliproteins.
The present study aimed to assess not only the efficacy of sap from two seaweeds Kappaphycus alvarezii (K-sap) and Gracilaria edulis (G-sap) on productivity and quality of Zea mays under rain-fed condition, but also to quantify whether sap application is beneficial in terms of lowering the carbon and phosphate footprint of mineral fertilizers per unit of produce. Field experiment was carried out to test 18 treatments, viz., 5 concentrations (2.5, 5.0, 7.5, 10 and 15 %) each of K-sap and G-sap applied along with recommended rate of fertilizers (RRF); 3 concentrations (7.5, 10 and 15 %) of each of the two types of sap applied along with 50 % RRF; alongside 2 control treatments T1 (water spray along with 100 % RRF) and T18 (water spray along with 50 % RRF). The optimal treatments that enhanced the grain productivity of maize were 5 % G-sap or 7.5 % K-sap applied in conjunction with 100 % RRF and the grain yield enhancements ranged from 21.4 to 29.8 % as compared to T1. Significant increase in P (35.5 %) and K (14.4 %) content in grains was observed through G-sap application, suggesting bio-stimulation in absorption of these elements. Notably, stover yield production at reduced RRF in certain combinations with sap was at par with T1 suggesting a possible saving on fertilizer requirement for fodder production under rain-fed conditions. Compared to T1, there was marked reduction of 17.5 and 23.1 % in global warming potential per unit of produce when 7.5 % K-sap and 5 % G-sap were used respectively in conjunction with 100 % RRF.
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