No abstract

Aims: The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species AD targeting the 5 0 untranslated region (5 0 UTR) of enteroviruses genome. Methods and Results: The RNA was converted to cDNA by the reverse transcriptase and then amplified via LAMP by the WarmStartâBst DNA polymerase, simultaneously in a single reaction tube, so we shortened the reaction time to 50 min. The sensitivity of the assay regarding Enterovirus B, C and D was determined to be 0Á30 CCID 50 assay À1 while the sensitivity for Enterovirus A was 3Á00 CCID 50 assay À1. The assay demonstrated high specificity and sensitivity for the detection of 45 reference strains of Enteroviruses AD and validated on 20 clinical isolates. Conclusions: This assay can be used as a diagnostic tool for the rapid, sensitive and specific detection of enteroviruses, easily implemented in small clinical and research laboratories since LAMP amplicons were visualized by colour changes eliminating the requirement for post-amplification processing steps. Significance and Impact of the Study: We developed a colorimetric assay ideal for field situations for the detection of enteroviruses, by targeting the 5 0 UTR. This assay demonstrated high specificity and sensitivity, based on its performance on 45 EV AD reference strains, on 20 EV B clinical isolates and on three non-enteroviral RNA viruses.

No abstract

No abstract

No abstract