ABSTRACT:Genetic diversity between 110 individuals from 6 elm populations (Ulmus minor, U. glabra and U. laevis) was determined using ISSR markers. Altogether 73 ISSR markers were evaluated with the average rate of polymorphic bands of 99.1%, which indicates high genetic diversity between the populations/species. The higher genetic diversity was revealed particularly in the population of U. glabra and this result was supported by the analysis of genetic diversity and differentiation of elm populations. Molecular analyses of ISSR markers allowed to assess the extent of genetic variability of native elm populations and characterize the levels of their genetic diversity and differentiation. Their further use can be seen in conservation and management activities.