Natural biomedical metabolites procured from marine sources have been the sole source of recent research. The antimicrobial resistance to human infections has made a mounting need for novel natural antibiotics. Much explored marine invertebrates largely the echinoderms (sea stars) tend to pose a natural innate mechanism to safeguard them against predators. The sea star secretes antimicrobial peptides which are naturally occurring secondary metabolites that possess a broad spectrum of antimicrobial susceptibility against bacteria, fungi, and viruses. The present study centers on the aspects of isolation and evaluation of active bioactive metabolite from the echinoderm Stellaster equestris from the Chennai coast. The whole body was utilized for the extraction using non-polar to polar solvents. The active crude extracts were investigated by qualitative assay for their chemical composition and were purified by column chromatography. The purity of the compound was further analyzed and checked for purity and quantified by the High-pressure liquid chromatography. The defined concentration of the isolated and purified compound from sea star Stellaster equestris (50,100, 150, 200, 250 and 300µg/ml) were subjected for antimicrobial sensitivity by well diffusion method and Tube dilution method. The outcome of the present study indicated the active crude extract from the sea star Stellaster equestris was rich in sterols. The evaluation of antimicrobial susceptibility by tube dilution and well diffusion assay indicated that the isolated purified compound from the sea star Stellaster equestris was reported to be evident for all the above-mentioned concentrations by a marked zone of clearance. A dose-dependent increase was observed in the tube dilution method. Therefore compounds possess antimicrobial activity and can be further subjected for developing the compound as a potent antimicrobial drug.