RESUMENCeratozamia mexicana Brongniart es una especie estrictamente dioica con un largo estadio juvenil. Con el fin de detectar marcadores moleculares asociados al azar (RAPD) y al análisis masal segregante (BSA), se trabajó con muestras de tejido foliar de individuos maduros de la población de esta especie en Coacoatzintla, Veracruz, México. Se usaron cinco iniciadores para amplificar tres mezclas de ADN de individuos de sexo conocido. Se produjeron un total de 37 bandas de las cuales el 63 % fueron polimórficas. Los iniciadores OPB08 y OPK10 generaron bandas distintivas al sexo. Con el iniciador OPK10 se observó una banda de 500 pb en dos de las tres mezclas de machos analizados, mientras que con el iniciador OPB08 se detectaron 2 bandas (1,400 y 1,100 pb) presentes en dos de las tres mezclas de machos examinados. Aunque es necesario validar los resultados obtenidos, pueden abrir una nueva alternativa para determinar tempranamente el sexo en C. mexicana. ABSTRACTCeratozamia mexicana Brongniart is a strictly dioecious species with a long juvenile stage. In order to detect molecular markers associated with sex, it was analyzed by the technique of random amplified polymorphic DNA (RAPD) and masal segregant analysis (BSA) samples of leaf tissue of leaf tissue of sexually differentiated individuals of the population of this species located at Coacoatzintla, Veracruz, México. 5 primers were used to amplify three bulk DNA samples of five known individuals from each sex. A total of 37 bands were produced of which 63% were polymorphic. The OPB08 and OPK10 primers generated distinctive bands to male sex in C. mexicana. With the OPK10 primer a band of 500 bp was observed in two of the three male bulk samples tested, while the OPB08 primer detected two bands (1400 and 1100 bp) in the male samples tested. Although it is necessary to validate this observation, these results could open a new alternative to early determination of sex in C. mexicana.
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