The sperm count has been correlated with the concentration of different substances secreted by the adnexal glands with conflicting results.In order to assess their diagnostic significance in male infertility the following substances were measured in seminal plasma: fructose and ascorbic acid produced by seminal vesicles and acid phosphatase and citric acid by the prostate gland. A statistical evaluation was made with the data obtained from azoospermic, oligozoospermic and control subjects.