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n experiment was conducted to investigate the effects of increasing dietary levels of organic chromium, organic selenium and vitamin E for deleterious impacts of heat stress on broilers. A total of 120 1-d old commercial broiler chicks (Cobb) were obtained from a local hatchary. Upon arrival the chicks were divided into four groups with six replications, each of five chicks. The first group was the control group, while the second group was fed the basal diet supplemented with organic chromium (0.8 mg/kg diet), the third group was fed the basal diet supplemented with organic selenium (0.3 mg/kg diet) and the fourth group was fed the basal diet supplemented with vitamin E (200 IU/kg diet).Body weight (BW); body weight gain (BWG); feed intake (FI) and feed conversion ratio (FC) were significantly (P≤0.05) improved in treatment groups compared to the control at 21 and 42 days of the age. Red blood cell count (RBSc), hematocrit (HT), hemoglobin (Hb), hemoglobin mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hemoglobin mean corpuscular volume (MCV) and heterophils lymphocytes ratios (H/l ratio) were significantly (P≤0.05) different in vitamin E; organic selenium and organic chromium compared to the control at 21 and 42 days of the age, but RBSc count at 21 days of age were not significant. Moreover, it has been noticed a significant (P≤0.05) increase occurrence in values of glutathione peroxidase (GPX), Super-oxide dismotase (SOD) and catalase (CAT) but the level of malondiadehyde (MDA) was significantly (P≤0.05) decreased by supplementing broiler diets with vitamin E; organic selenium and organic chromium, respectively compared to the control. Supplementing broiler diets with vitamin E; organic selenium and organic chromium improved (P≤0.05) the carcass characteristics, respectively compared to the control. However, these improvements were insignificant in abdominal fats, thymus, bersa and spleen. The present results indicate that the supplementation of diets with vitamin E; organic selenium and organic chromium, especially vitamin E, is necessary to overcome the deleterious effects of heat stress on broilers.

ne hundred and twenty 1-d old commercial broiler chicks (Cobb) were divided in to four groups with six replicats, to investigate the effect of dietary supplementation with organic chromium (Cr), organic selenium (Se) and Vitamin E (Vit.E) on some physiological and immunological parameters. The first group was the control group, while the second group was fed the basal diet supplemented with organic chromium (0.8 mg/kg diet), the third group was fed the basal diet supplemented with organic selenium (0.3 mg/kg diet) and the fourth group was fed the basal diet supplemented with vitamin E (200 IU/kg diet). Temperature and humidity were recorded daily and the Temperature-Humidity Index (THI) was calculated, which demonstrated that birds were exposed to heat stress throughout the experiment period. The results showed that increasing dietary levels of organic chromium, organic selenium and Vitamin E especially Vitamin E supplementation could improve plasma total protein and globulin (p<0.05) values at 42 DOA. Supplements could significantly (p<0.05) enhance the total antibody titer against Newcastle disease Virus (NDV) either for the primary or secondary response and induce higher expression of heat shock protein 70 (HSP 70). Insulin-like Growth factor-I (IGF-I) level was improved significantly (p<0.05) due to increasing dietary organic chromium levels followed by vitamin E than organic selenium. It could be concluded that the dietary addition of organic chromium (Cr), organic selenium (Se) and Vitamin E (Vit.E) triggered improvements in the physiological and immunological measurements of broilers reared under heat stress conditions

n experiment was conducted to investigate the effects of increasing dietary levels of organic chromium, organic selenium and Vitamin E on heat stressed broilers. A total of 120 1-d old commercial broiler chicks (Cobb) were obtained from a local hatchary. Upon arrival the chicks were divided into four groups with six replications, each of five chicks. The first group was the control group, while the second group was fed the basal diet supplemented with organic chromium (0.8 mg/kg diet), the third group was fed the basal diet supplemented with organic selenium (0.3 mg/kg diet) and the fourth group was fed the basal diet supplemented with vitamin E (200 IU/kg diet).Body weight (BW); body weight gain (BWG); feed intake (FI) and feed conversion ratio (FC) were significantly (P≤0.05) improved in treatment groups compared to the control at 21 and 42 days of the age. Red blood cell count (RBSc), hematocrit (Ht), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV) and heterophils/lymphocytes ratios (H/l ratio) were significantly (P≤0.05) different in vitamin E; organic selenium and organic chromium compared to the control at 21 and 42 days of the age, but RBSc count at 21 day of age were not significant. Moreover, values of glutathione peroxidase (GPX), Super-oxide dismutase (SOD) and Catalase (CAT) were significantly (P≤0.05) increased but Malondiadehyde (MDA) was significantly (P≤0.05) decreased with supplemental vitamin E; organic selenium and organic Chromium compared with control. Carcass characteristics of supplemented broilers were also improved (P≤0.05) compared to the control. However, abdominal fat, thymus, bursa and spleen were not significantly changed. The present results indicate that dietary supplementation of vitamin E; organic selenium and organic chromium, especially vitamin E, is necessary to overcome the deleterious effects of heat stress on broilers.