The need to economic, sensitive and easily maintained cell cultures use for massive production of fowl pox vaccine was necessary. We adapted Fowl pox virus (FPV) Baudette strain on Primary chicken embryo fibroblast (CEF), Baby Grivet Monkey Kidney cell line (BGM) and African green monkey kidney (Vero) cell lines. The highest virus titers were 10 6.5 after the 9 th passage in CEF and 10 6.2 after the 12 th passage on BGM while Vero cells were abortive to the virus. BGM cells were selected for study cytopathic effect (CPE) and growth kinetic as it easily maintained than primary CEF cells. The characteristic CPE were rounding till 6 th passage with intracytoplasmic inclusion by the 7 th passage and syncytium formation appeared from the 9 th passage till the 13 th passage. The virus was highly cell associated for the first 84h post inoculation(PI) with a titer of 10 4.5 then a maximum titer was 10 6.2 in cell free portion after 120h PI. In conclusion, BGM were considered a new susceptible cell for growth of FPV with best harvesting time 120h PI to obtain a maximum titer for subsequent vaccine production.

Aiming to raise the levels of immunity induced in dogs by the cell culture inactivated rabies vaccine, bee venom BV was subjected to investigate its immune stimulant effect in vaccinated dogs. It was found that a dose of 1mg of BV/ dog did not cause any post inoculation reaction showing its safety. Mutual vaccination of dogs with BV inoculation was carried out in different groups of susceptible dogs of about 3-5 months' age. Monitoring of the exhibited rabies antibodies in vaccinated dogs using serum neutralization test (SNT) and indirect Enzyme Linked Immune Sorbent Assay (ELISA) revealed that BV induced the highest levels of antibodies (128 by SNT and 7 log2 and 6 log2 by ELISA) when inoculated before and simultaneously with rabies vaccine. Rabies vaccine alone or before inoculation of BV induced lower titers of antibodies (32&64 and 5 & 6log2 by SNT and ELISA respectively) by the 4 th week post vaccination. However, BV could be used to initiate the immune response of dogs to rabies vaccine.