In nature, microorganisms including fungi are the most efficient cellulose and hemicelluloses degraders through production of lignocellulosic enzymes. Termitomyces spp., a wild edible mushroom is well known for production of lignocellulosic enzymes. Twenty isolates of Termitomyces spp. were isolated at 10 -4 dilution with pour plate method from different locations of termite mound soil of Tamil Nadu. Based on their morphological and cultural characteristics, the isolates were identified as Termitomyces. Growth conditions and cultural characteristics of Termitomyces spp. isolates were studied. The results showed that the isolates grew well in Malt extract agar medium with pH 5.5 when incubated at 27 o C for 7 days. The isolates were screened for the activity of enzymes e.g., cellulase, amylase, xylanase, polyphenol oxidase and peroxidase activities at seven different incubation periods. Maximum activity of the enzymes was observed on 12 th day after inoculation which declined thereafter.