The study aimed to evaluate the use of lactic acid as an acidifier in rations containing probiotics on blood fat profile of sentul chicken. Sixty female Sentul chickens aged 6 months were reared for two months in a 20-unit battery cage and subjected to a completely randomized design (CRD) with four treatments and five replicates, with 3 females for each unit. The treatments were R0 : Probiotic basal rations (BS); R1 : BS + 0.5% lactic acid; R2 : BS + 1.0% lactic acid; and R3 : BS + 1.5% lactic acid. Data were analyzed by Analisis of Variance (ANOVA) using an IBM SPSS Statistics ver. 22. The result showed that lactic acid as an acidifier in rations containing probiotics did not significantly (P>0,05) affected blood fat profile (cholesterol, HDL, LDL and triglycerides) of Sentul chicken. The average cholesterol levels were 112.77±26.55 mg/dl; HDL 40.73±9.53 mg/dl; LDL 41.32±24.39 mg/dl; and triglycerides 153.57±46.38 mg/dl. The conclusion was that the use of up to 1.5% lactic acid as an acidifier in feed created similar results in cholesterol, HDL, LDL and triglycerides. Keywords: Sentul chicken, blood fat, acidifier