The aim of this study was to identify the polymorphism of the CD1B gene in c.248 in exon 3 and its association with IgY and ND titers in sentul chicken Sensi-1 Agrinak line. IgY is the main antibody of chickens while the ND titer is an ND specific antibody, both of which have an important role in the response of disease resistance. The Cluster of differentiation 1B (CD1B) gene is one of the major genes of disease resistance that functions in representing antigens through specific immune responses. This study used 17 sensi-1 chicken lines. The study was conducted by PCR sequencing method, ELISA test, and HI test. The result showed mutation on c248 A>G in exon 3. The SNP were polymorphic and Hardy Weinberg equilibrium. Thre were two A and G alleles and 3 genotypes namely AG, GG, and AA. The frequency of the GG genotype was higher (0.41) compared to other genotypes. The AG genotype were associated with IgY production and were not associated with ND titers.

The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G>A, c.562 T>A, c.588 A>G, and c.612 C>G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G>A and c.562 T>A SNPs were in Hardy Weinberg's equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A>G and c.612 C>G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e.

The concentration of IgY in the body is often associated with resistance to bacterial infections, whereas ND specific antibody titters are indicated as resistance to viral infections. The aim of this study was to determine the characteristics of IgY and ND titters in newly released chickens, and to compare them with native chickens spread in the community. This research used 286 IPB-D1 chickens, Jatiwangi Sentul chickens, and Sensi-1 chicken. IgY concentration were analyzed using the ELISA method, and ND titters were analyzed using the HI test. The results of the study showed high IgY concentrations were found in IPB-D1 chickens (10.10) and Sensi-1 chickens (10.63), while moderate IgY concentrations were found in jatiwangi sentul chickens (8.63). The results of ND titters showed that the chicken IPB-D1 (2.08) and Sensi-1 chicken (2.54) were higher than Sentul Jatiwangi chickens (0.70). It could be concluded that IPB-D1 chickens and Sensi-1 chickens are more resistant to disease infections than other chickens. The disease resistance selection program in Sentul Sensi-1 chickens, and the utilization of the heterosis effect through the crossing program in chickens IPB-D1 has been shown to increase good disease resistance indicators. Selection and crossbreeding programs in IPB-D1 and Sensi-1 chicken have been proven to increase resistance characteristics.