The study aimed at assessing the knowledge and socio-demographic determinants on occupational hazard prevention among automobile mechanics in Akure South Local Government (AKSLG), Ondo State, Nigeria. Methods: Descriptive cross-sectional design was utilized with automobile mechanic serving as the study population. Respondents were selected by stratified random sampling technique. The study population included 231 respondents from 13 units within the AKSLG. Self-developed questionnaire was administered for data collection. Data was analyzed using descriptive statistics, Pearson correlation and Regression model at p < 0.05 Results: Participants' age ranged between 20-50 years and majority had above 15 years of experience with monthly income above ? 58,000 ($161). The findings revealed that most (62.0%) of the respondents had fair knowledge of occupational hazard. Also, 38.0% of the respondents had poor knowledge on the prevention of occupational hazard. Significant relationship was found between years of experience; knowledge about occupational hazard prevention and occupational hazard prevention among respondents with p-values of 0.005 and 0.007 respectively. Conclusion: Socio-demographic variable of years of experience and knowledge of automobile mechanics about occupational hazards prevention had significant relationship on prevention of occupational hazards, therefore measures to enhance knowledge about occupational hazards should be implemented to improve occupational hazard prevention among automobile mechanics.