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Sodium dodecyl sulphate-acrylamide gel electrophoresis (SDS-AGE) separates proteins according to their molecular weight (MW) without being influenced by their electrostatic charge. It was carried out on the urine of 17 healthy control subjects end 92 ambulatory patients with suspected or known renal impairment. The SDS-AGE patterns were classified as physiological, low NW predominance, middle M3V predominance, high bfW predominance, or mixed low and high MW. Patients were separately classified as having either normal kidneys or glomerular, tubular or mixed renal lesions according to the results of clinical investigation. Comparison of both claesificatione revealed that SDS-AGE allowed a correct diagnosis of the site of renal involvement in A3 per cent of cases. Predominantly low MW protein excretion correlated with tubular damage. Middle and high M\;V patterns correlated with glomerular disease, and a relationship between nephrotic syndrome with minimal glomerular changes end middle M3V pattern was found in two caaee, suggesting that SDS-AGE could be used to evaluate the selectivity of prohinuria.However, the estimation of selectivity by SDS-AGE should be studied in a sufficient number of cases and compared with measurements of selectivity by the protein clearance method.

Sodium dodecyl sulphate-acrylamide gel electrophoresis (SDS-AGE) separates proteins according to their molecular weight (MIL') without being influenced by their electrostatic charge. It was carried out on the urine of 17 healthy control subjects and 92 ambulatory patienta with suspected or known renal impairment. The SDS-AGE patterns were classified as physiological, low MW predominance, middle MJV predominance, high MW predominance. or mixed low and high M W . Patienta were separately classified ne having either normal kidneys or glomerular, tubular or mixed renal lesions according to the results of clinical investigation. Comparison of both classifications revealed that SDS-AGE allowed a correct diagnosis of the site of renal involvement in 83 per cent of cases. Predominantly low MW protein excretion correlated with tubular damage. Middle and high MW patterns correlated with glomedar disense, and a relationship between nephrotic syndrome with minimal glomerular changes and middle MW pattern was found in two cases, suggesting that SDS-AGE could be used to evaluate the selectivity of proteinuria. However, the eatimtation of selectivity by SDS-AGE should be studied in a sufficient number of cases and compared with measurements of selectivity by the protein clearance method.