The resin-dentin interdiffusion zone produced by a dentin-adhesive system that removes the smear layer and concurrently decalcifies superficial dentin was morphologically examined by both scanning and transmission electron microscopy. Cross-sectioned resin-bonded dentin discs were etched with an argon-ion beam to make the resin-dentin interface observable by SEM. For the TEM examination, the sections were partly decalcified by an aqueous EDTA solution to facilitate ultramicrotomy and to disclose the ultrastructure of the interdiffusion zone. Both SEM and TEM confirmed the presence of the resin-dentin interdiffusion zone as the junction between the deep unaltered dentin structure and the restorative resin. Within the interdiffusion zone, three sublayers with characteristic ultrastructure and staining were identified by TEM. An upper diffuse black layer contained few structural features. Underneath, partially-altered collagen fibrils were closely packed, mostly running parallel with the interface and perpendicular to the dentinal tubules. Their outline was electron-dense, forming tunnel-like structures. At the base of the upper layer, several stained projections were found to bulge out into the underlying collagen network and appeared to be confined by obstructive, parallel-running collagen fibrils. Finally, the third dense layer, containing hydroxyapatite crystals, demarcated the superficially demineralized dentin layer from the deeper unaltered dentin. Resin diffusion into the decalcified dentin surface layer was evident, but diminished with depth, presumably reducing deeper resin impregnation into the interfibrillar spaces. The citric acid dentin-pretreatment probably caused denaturation of the superficial collagen fibrils. Its decalcifying effect gradually weakened with depth, leaving behind hydroxyapatite crystals at the base of the interdiffusion zone.(ABSTRACT TRUNCATED AT 250 WORDS)

The mandibular symphysis studied by histology and microradiography in the fetus and in the newborn shows a mineralized tissue different from the calcified cartilage observed in endochondral ossification and also different from bone aspects. In 1888, Schaffer coined it ‘chondroid bone’, but this tissue derives from secondary cartilage. We therefore suggest the term ‘chondroid tissue’ to designate it.

This paper deals with investigations concerning the matrix of chondroid tissue. Among the 73 human fetus or child mandibles and the 42 cat mandibles we have studied histologically and microradiographically, 8 human and 3 cat mandibles were used to determine the collagen composition in chondroid tissue matrix, and 10 cat mandibles were analyzed in order to have an ultrastructural approach to chondroid tissue. Both in human and cat mandibles, types I and II collagen were identified by indirect immunofluorescence and immunoperoxidase techniques. Electron microscopic analysis shows large collagen fibrils which correspond to type I collagen, and smaller collagen fibrils, principally located at the periphery of the chondroid cells. From our investigations, chondroid tissue should be considered as being different from both bone and cartilage, although it is not a transitional tissue, since no transformation of chondroid tissue into bone is observed; it should be classified as an intermediate tissue between cartilage and bone because of its morphological characteristics.

The cranial vault of fifteen human subjects varying in age from 20th week of gestational life to 9th month post-matum were submitted to microradiographic and histological analysis. Different phenomena such as cortical drift, bone cavitation and progressive substitution of different calcified tissues by lamellar bone are illustrated. Moreover, this study reveals in several areas the presence of chondroid tissue; it constitutes the edges of the sutures and is responsible for their growth till the post-natal period. Therefore, it can be supported that the role of chondroid tissue is essential for the harmonious development of the cranial vault.

-The aims of this study were (1) to correlate cough and body temperature (BT) with the severity of bronchopneumonia in pigs, (2) to determine whether these clinical signs can be used to early diagnose bronchopneumonia and (3) to assess the predictive values of cough and BT regarding lung lesions. Bronchopneumonia was induced by administering E. coli endotoxin (LPS) combined with Pasteurella multocida type A (PmA) in the trachea of 13 piglets. Saline-instilled negative controls (n = 8), PmA inoculated (n = 6) and LPS instilled (n = 5) groups were also constituted. Cough and BT were recorded daily while the bronchopneumonia severity was assessed using bronchoalveolar lavage fluid (BALF) cytology, cytokines and measurement of lung lesion volume. Changes in expiratory breathing pattern were also measured (Penh). The combination of LPS and PmA induced a subacute bronchopneumonia characterised by macrophage, neutrophil, and lymphocyte infiltration, changes in Penh and an increase in the mRNA level of IFN-γ while IL8, IL-18 and TNF-α mRNA levels remained unchanged. The daily body weight gain of infected animals was significantly reduced. Cough and BT changes were proportional to the intensity of the lung inflammatory process, functional respiratory changes and to the extent of macroscopic lesions. When comparing the individual values of cough and BT to thresholds defined for both parameters, an early diagnosis of pneumonia was possible. Considering the pooled data of each group, it was possible to define thresholds allowing an early segregation between the groups of diseased and healthy piglets. The daily values of cough and BT were predictive for the volume of lung lesions recorded at the end of the trial. In conclusion, cough and BT appear as potential indicators for the intensity and the evolution of the respiratory disease. They also seem to be good predictors for the magnitude of lung lesions and weight gain recorded at the study endpoint.