New 2-Mercaptobenzimidazole derivatives were synthesized. 4,5-disubsitituted 1,2,4-Triazole compounds 1b-2c were synthesized from 2-(benzylthio) benzimidazole compound a, which was then reacted with (NaH) in dioxane at a temperature of (0-5 C°) to produce the salt of compound a. Then the salt was reacted with ethyl chloro acetate to yield Ethyl 2-(benzylthio) benzimidazole acetate compound b. Compound b was converted to triazole derivatives by two pathways. The first pathway was reacting compound b with semicarbazide, thiosemicarbazide and phenylsemicarbazide in DMSO as a solvent to gain compounds 1b-3b, which were then cyclized by refluxed with 2N (NaOH) to yield 1,2,4-triazole derivatives compounds 4b-6b. The second pathway involved the treatment of compound b with hydrazine hydrate to produce N-acetohydrazide-2-(benzyl thio) benzimidazole c. Compound c was refluxed with carbon disulfide (CS2) in KOH alcoholic solution to obtain the salt compound 1c. The salt was treated with hydrazine hydrate to yield 1,2,4-triazole derivative compound 2c. The newly synthesized compounds b-2c were identified by FTIR, 1H-NMR and 13C-NMR and their physical properties were measured. Furthermore, their anti-microbial activities were tested against two Gram-positive and two Gram-negative bacteria and against one strain of fungi. Also, some of these synthesized compounds were tested for their anti-inflammatory activities.

This research includes preparing of some substituted 1,2,4-triazoles and 1,3,4thiadiazoles through the reaction of each of phthalimide and cis-1,2,3,6-tetrahydrophthalimide with ethyl bromo acetate in the presence of sodium hydroxide, and absolute ethanol to obtain the esters ethyl phthalimide acetate, then converted these esters to thiosemicarbazide and semicarbazide derivatives through their reaction with thiosemicarbazide and semicarbazide respectively. Furthermore, substituted triazole were prepared through the cyclization of thiosemicarbazide and semicarbazide in alkaline media such as sodium hydroxide solution, also thiadiazole through the treatment of esters, ethyl phthalimide acetate and ethyl cis-1,2,3,6tetrahydrophthalimide acetate with thiosemicarbazide in the presence of a base. The prepared compounds were identified by spectral methods FTIR, 1 H-NMR, 13 C-NMR, and measurements some of its physical properties and some specific reactions, furthermore we were studied the effects of the preparing compounds on some strains of bacteria, Staphylococus aureus,Escherichia Coli, Serrtia, which exhibited antibacterial activity against Escherichia Coli specifically and have no effect on other strains.