This study addresses the extent to which insecure and disorganized attachments increase risk for externalizing problems using meta-analysis. From 69 samples (N = 5,947), the association between insecurity and externalizing problems was significant, d = 0.31 (95% CI: 0.23, 0.40). Larger effects were found for boys (d = 0.35), clinical samples (d = 0.49), and from observation-based outcome assessments (d = 0.58). Larger effects were found for attachment assessments other than the Strange Situation. Overall, disorganized children appeared at elevated risk (d = 0.34, 95% CI: 0.18, 0.50), with weaker effects for avoidance (d = 0.12, 95% CI: 0.03, 0.21) and resistance (d = 0.11, 95% CI: -0.04, 0.26). The results are discussed in terms of the potential significance of attachment for mental health.

This meta-analysis included 66 studies (N = 4,176) on parental antecedents of attachment security. The question addressed was whether maternal sensitivity is associated with infant attachment security, and what the strength of this relation is. It was hypothesized that studies more similar to Ainsworth's Baltimore study (Ainsworth, Blehar, Waters, & Wall, 1978) would show stronger associations than studies diverging from this pioneering study. To create conceptually homogeneous sets of studies, experts divided the studies into 9 groups with similar constructs and measures of parenting. For each domain, a meta-analysis was performed to describe the central tendency, variability, and relevant moderators. After correction for attenuation, the 21 studies (N = 1,099) in which the Strange Situation procedure in nonclinical samples was used, as well as preceding or concurrent observational sensitivity measures, showed a combined effect size of r(1,097) = .24. According to Cohen's (1988) conventional criteria, the association is moderately strong. It is concluded that in normal settings sensitivity is an important but not exclusive condition of attachment security. Several other dimensions of parenting are identified as playing an equally important role. In attachment theory, a move to the contextual level is required to interpret the complex transactions between context and sensitivity in less stable and more stressful settings, and to pay more attention to nonshared environmental influences.

No abstract

In a longitudinal study, internationally adopted children (N ϭ 146) placed before 6 months of age were followed from infancy to age 7. Results showed that girls were better adjusted than boys, except in cognitive development, and that easy temperament was associated with higher levels of social, cognitive, and personality development and fewer behavior problems. Higher quality of child-mother relationships, in terms of attachment security and maternal sensitivity, uniquely predicted better social and cognitive development. The combination of attachment disorganization and difficult temperament predicted less optimal ego-control and lower levels of cognitive development. It is concluded that even in adopted children, who are not biologically related to their adoptive parents, early mother-infant interactions and attachment relationships predict later socioemotional and cognitive development, beyond infant temperament and gender.

Characterizing genetic influences on DNA methylation (DNAm) provides an opportunity to understand mechanisms underpinning gene regulation and disease. In the present study, we describe results of DNAm quantitative trait locus (mQTL) analyses on 32,851 participants, identifying genetic variants associated with DNAm at 420,509 DNAm sites in blood. We present a database of >270,000 independent mQTLs, of which 8.5% comprise long-range (trans) associations. Identified mQTL associations explain 15-17% of the additive genetic variance of DNAm. We show that the genetic architecture of DNAm levels is highly polygenic. Using shared genetic control between distal DNAm sites, we constructed networks, identifying 405 discrete genomic communities enriched for genomic annotations and complex traits. Shared genetic variants are associated with both DNAm levels and complex diseases, but only in a minority of cases do these associations reflect causal relationships from DNAm to trait or vice versa, indicating a more complex genotype-phenotype map than previously anticipated.(Extended Data Fig. 5). These results show the value of large sample sizes in blood to detect trans-mQTLs regardless of the tissue. Trans-mQTL SNPs and DNAm exhibit patterned TF binding.Recent studies have uncovered multiple types of transcription factor (TF)-DNA interactions influenced by DNAm, including the binding of DNAm-sensitive TFs [26][27][28] and cooperativity between TFs 27,29 . To gain insights into how SNPs induce long-range DNAm changes, we mapped enrichments for DNAm sites and SNPs across binding sites for 171 TFs in 27 cell types 30,31 . We found strong enrichments for most TFs and cell types among DNAm sites with a trans association (cis + trans: 55%; trans only: 80%; cis only: 18%) and among cis-acting SNPs (cis only: 96%, cis + trans: 91%, trans only: 1%; Fig. 2b, Supplementary Tables 7 and 8, and Supplementary Figs. 22 and 23). Consistent with the observation that trans-only DNAm sites are enriched for CpG islands (Supplementary Fig. 13), DNAm sites that overlap TF-binding sites (TFBSs) were relatively hypomethylated (weighted mean DNAm levels = 21% versus 52%, P < 2.2 × 10 −16 ; Supplementary Fig. 24).Next, we hypothesized that, if a trans-mQTL is driven by TF activity 8,10 , then particular TF-TF pairs may exhibit preferential enrichment 32 . An mQTL has a pair of TFBS annotations 31 , one for the SNP and one for the DNAm site. We evaluated whether the annotation pairs among 18,584 interchromosomal trans-mQTLs were associated with TF binding in a nonrandom pattern (Supplementary Note and Extended Data Fig. 6a,b). We found that 6.1% (22,962 of 378,225) of possible pairwise combinations of SNP-DNAm site annotations were more over-or underrepresented than expected by chance after strict multiple testing correction (Supplementary Note, Supplementary Table 9 and Extended Data Fig. 6c).After accounting for abundance and other characteristics, the strongest pairwise enrichments involved sites close to TFBSs for proteins in the cohesin complex, ...