During development, cortical plasticity is associated with the rearrangement of excitatory connections. While these connections become more stable with age, plasticity can still be induced in the adult cortex. Here we provide evidence that structural plasticity of inhibitory synapses onto pyramidal neurons is a major component of plasticity in the adult neocortex. In vivo two-photon imaging was used to monitor the formation and elimination of fluorescently labeled inhibitory structures on pyramidal neurons. We find that ocular dominance plasticity in the adult visual cortex is associated with rapid inhibitory synapse loss, especially of those present on dendritic spines. This occurs not only with monocular deprivation but also with subsequent restoration of binocular vision. We propose that in the adult visual cortex the experience-induced loss of inhibition may effectively strengthen specific visual inputs with limited need for rearranging the excitatory circuitry.
During critical periods of development, experience shapes cortical circuits, resulting in the acquisition of functions used throughout life. The classic example of critical-period plasticity is ocular dominance (OD) plasticity, which optimizes binocular vision but can reduce the responsiveness of the primary visual cortex (V1) to an eye providing low-grade visual input. The onset of the critical period of OD plasticity involves the maturation of inhibitory synapses within V1, specifically those containing the GABA receptor α1 subunit. Here we show that thalamic relay neurons in mouse dorsolateral geniculate nucleus (dLGN) also undergo OD plasticity. This process depends on thalamic α1-containing synapses and is required for consolidation of the OD shift in V1 during long-term deprivation. Our findings demonstrate that thalamic inhibitory circuits play a central role in the regulation of the critical period. This has far-reaching consequences for the interpretation of studies investigating the molecular and cellular mechanisms regulating critical periods of brain development.
In adult primary visual cortex (V1), dendritic spines are more persistent than during development. Brain-derived neurotrophic factor (BDNF) increases synaptic strength, and its levels rise during cortical development. We therefore asked whether postsynaptic BDNF signaling through its receptor TrkB regulates spine persistence in adult V1. This question has been difficult to address because most methods used to alter TrkB signaling in vivo affect cortical development or cannot distinguish between pre-and postsynaptic mechanisms. We circumvented these problems by employing transgenic mice expressing a dominant negative TrkB-EGFP fusion protein in sparse pyramidal neurons of the adult neocortex and hippocampus, producing a Golgi-staining-like pattern. In adult V1, expression of dominant negative TrkB-EGFP resulted in reduced mushroom spine maintenance and synaptic efficacy, accompanied by an increase in long and thin spines and filopodia. In contrast, mushroom spine maintenance was unaffected in CA1, indicating that TrkB plays fundamentally different roles in structural plasticity in these brain areas.adult cortical plasticity ͉ BDNF signaling ͉ synapse stability ͉ transgenic mice D uring development, synapse formation and elimination are regulated by molecular cues, spontaneous activity, and experience (1, 2). Most glutamatergic synapses on excitatory neurons are situated on dendritic spines. Live imaging of neurons expressing GFP has provided important information on the dynamics of spine formation and maintenance (3-8). Filopodia are short-lived fingershaped protrusions and believed to be precursors of dendritic spines (9, 10). Newly formed spines are often thin or long and appear and disappear within days. Some mature into mushroom or stubby spines, which are more stable and often persist for months (7,8). There are strong correlations between spine size, spine persistence, synaptic efficacy, and the number of ␣-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) at the postsynaptic density (8,11,12). With development and aging of the cortex, there is a shift toward larger and more persistent spine types (3,7,13).Spine dynamics are influenced by plasticity. Long-term potentiation in hippocampus is associated with an increase in spine size (14) and spine formation (15), whereas term depression is associated with spine elimination (16). Interestingly, reducing synaptic input results in an increase in spine numbers, probably due to homeostatic mechanisms (17)(18)(19).Ocular dominance plasticity in V1 is associated with initial pruning and later formation and stabilization of spines (20,21) and occurs predominantly during a critical period of development. Maturation of the extracellular matrix is a major factor in ending the critical period, probably by increasing spine and axon stability (20)(21)(22) BDNF signaling through TrkB receptors is a key player in visual plasticity (23,24). It drives the development of inhibitory innervation, an important factor in ocular dominance plasticity (25,26). BDNF is...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.