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Esterase D: Simultaneous electrophoretic determination of the three common allozymes (ESD 1,2,5)A simple and timesaving electrophoretic method for the determination of the 6 common phenotypes of red cell esterase D (ESD) is presented. Using a continuous buffer system composed of malic acid, the separation of the allozymes is accomplished at pH-values between 5.35-5.45 by thin-layer agarose gel electrophoresis in 4 h. Until now, a combination of electrophoresis and isoelectric focusing was required to obtain the same results. The economical advantage as well as the modest requirements concerning laboratory equipment are evident.