It is essential to have a good knowledge of laser operation and of which type of laser is most appropriate for each lesion.

Comparison among the results of the studies is difficult due to the use of different indexes and diagnostic criteria, the analysis variability, selection methods, and different age groups. In reality, we are probably far from knowing the real MIH prevalence.

Background - Little information is available regarding the eruption chronology of Spanish children. Therefore, it is important to acquire accurate eruption parameters for the Spanish population. Aim - To establish the chronology of the permanent dentition. Design - A cross-sectional study based on a sample of 1123 Spanish Caucasian children ranging from 5 to 15 years of age, from three different schools in Barcelona, Spain. Results - The sample is representative of the Spanish population with regards to age, height and weight; and large enough to achieve the desired 95% confidence level. The results appear in the eruption tables included in the body of this paper. Conclusions - In both boys and girls, the lower left central incisor is the first, and the upper right second molar is the last tooth to erupt. Mandibular teeth tend to erupt before the corresponding maxillary teeth; however, there is no difference between the left and right sides of each arch. The results obtained are in agreement with similar studies performed in other groups of Caucasian children.

The present research reveals for the first time a statistically significant relationship between atopic dermatitis and food allergies with the presence of molar-incisor hypomineralization.

Objective: An insufficient mineralization (hypomineralization) in the teeth during the maturation stage of amelogenesis cause defects in 3-44% of children. Here, we describe for the first time the microbiota associated with these defects and compared it to healthy teeth within the same subjects. Methods: Supragingival dental plaque was sampled from healthy and affected teeth from 25 children with molar-incisor hypomineralization (MIH). Total DNA was extracted and the 16S rRNA gene was sequenced by Illumina sequencing in order to describe the bacterial composition. Results: We detected a higher bacterial diversity in MIH samples, suggesting better bacterial adhesion or higher number of niches in those surfaces. We found the genera Catonella, Fusobacterium, Campylobacter, Tannerella, Centipeda, Streptobacillus, Alloprevotella and Selenomonas associated with hypomineralized teeth, whereas Rothia and Lautropia were associated with healthy sites. Conclusion: The higher protein content of MIH-affected teeth could favour colonization by proteolytic microorganisms. The over-representation of bacteria associated with endodontic infections and periodontal pathologies suggests that, in addition to promote caries development, MIH could increase the risk of other oral diseases.