The aim: To determine the effect of solar activity on the cardiac rhythm indices of apparently healthy men and women, smokers and non-smokers, depending on the stress load (physical and psycho-emotional). Methods. The registration of myocardium bioelectric activity parameters was carried out in the participants of the experiment (26 people) for 2 years (spring, autumn, 95 days), using ECG sensor «Fazagraf», the cardiosignals were handled on-line (www.geliomed.kiev.ua). Long time-series data were mathematically processed using Microsoft Office Excel and STATISTICA programs. The change of geomagnetic storminess was estimated using Кр-index. Results. Biophysical monitoring data ofcardiac rhythm was analyzed. In accordance with the ergodic hypothesis, a statistical analysis of balance factor values of the T-wave (T) of ECG was performed using time series for 4 groups with common traces - gender and tobacco smoking. Using the Pearson's chi-squared test, it was proved that the samples obeyed the normal distribution law. Significant differences in the cardiac rhythm were revealed after physical and emotional loads in smokers in comparison with non-smokers, regardless of the geomagnetic situation. The mean values of the T-wave balance factor changes were calculated after the stress load relative to the rest state (ΔT) for the indicated groups of subjects, for periods with a quiet geomagnetic environment (Kp < 16) and with high geomagnetic activity (Kp > 16). Significant differences (р < 0.05) in the parameters of cardiac rhythm changes under the load in the magnetic disturbed days in comparison with the unperturbed period were found only in smokers. The greatest differences were recorded in women-smokers under psycho-emotional stress and in men smokers after physical activity. Conclusion. Physical load and psycho-emotional stress are risk factors for the cardiovascular system of smokers, respectively, in days with high sunspot activity.

The effect of the solvent nature (acetonitrile, ethanol, dimethyl sulfoxide, and dioxane) and its concentration on the fluorescence intensity of pyrene sorbed on the cellulose diacetate (CDA) film from a water-organic solution was studied. Dimethyl sulfoxide and ethanol are shown to be the most effective solvent additives for pyrene solid-surface fluorescence (SSF). The maximum SSF signal of pyrene was found upon sorption of the substance from aqueous media containing 1.2-4.2 vol% DMSO. For the pyrene quantitation the concentration dependence of its SSF intensity at the maximum of the spectrum at em = 394 nm and exp = 320 nm was plotted. The dependence has a linear character in the pyrene concentration range 2⋅10 −6 -2⋅10 −8 g/L, and the limit of pyrene detection is 2⋅10 −11 g/L. The possibility of determining benzo(a)pyrene using SSF technique with the CDA matrix is proved. The proposed method is promising for use in environmental monitoring of polycyclic aromatic hydrocarbons.

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Саратовский государственный технический университет имени Гагарина Ю. А. 2 Варшавский университет, Центр биологических и химических исследований, Польша 3 Саратовский национальный исследовательский государственный университет имени Н. Г. Чернышевского Исследованы процессы люминесценции ПАУ (антрацена, фенантрена, пирена, флуорантена, бенз(а)антрацена, хризена, бенз(а)пирена) в водных средах и в твердой фазе матрицы из диацетата целлюлозы после сорбции из данных сред. Установлено, что метод твердофазной люминесценции (ТФЛ) позволяет увеличить чувствительность определения ПАУ в воде без предварительной экстракции и концентрирования, исключение составляет хризен. Полученные спектральные характеристики позволяют полагать, что с помощью метода ТФЛ можно обнаруживать пары индикаторных изомеров ПАУ. Ключевые слова: полициклические ароматические углеводороды, твердофазная люминесценция, твердофазная экстракция, диацетат целлюлозы.