were stimulated by xylem fluids from the susceptible, but not from the resistant cultivars.A resistance response based on physical containment and reduced fungal growth was evident.
Meloidogyne pisi n. sp. is described and illustrated from specimens obtained from roots of pea cv. Mikado in Brasilia, Brazil. The female perineal pattern is variable in shape, most are rounded to ovoid, whereas others have a moderately high, squarish dorsal arch, and can be marked with several striae parallel to the vulva or may be nearly free of striae. Lateral fields may contain a few irregular, wavy, transverse striae or be difficult to discern. Female medial lips are wide and long, sometimes with distinctly indented outer margins; the lateral lips are large and triangular, head annule is wide and marked by incomplete annulations and transverse folds. Stylet is 13-17 µm long with large, elongated knobs and a shaft that is cylindrical, straight and thick. Lumen lining of pharynx containing numerous rounded cuticular thickenings throughout its length; excretory pore located between base of stylet and median bulb. Males are 893-2510 µm long with a 19-26 µm long stylet bearing large elongated knobs, shaft marked with small, rounded projections. Second-stage juveniles are 374-463 µm long with medial lips often indented medially and 10-11 µm long stylet. Tail slender, 42-66 µm long with large irregular annules in posterior region, a slightly pointed tip and hyaline region 9-17 µm long. Many galls and egg masses are produced on tomato, tobacco and varieties of pea and bean. Few galls occur on watermelon and corn and reproduction is poor on pepper. No reproduction occurs on peanut, cotton or soybean.Pea plants (Pisum sativus L.) collected from the Nucleo Rural do Jardim area of Brasilia, Brazil, were severely infected with root-knot nematodes. Symptoms included chlorosis, stunting, root rot, and a complete lack of nodulation by Rhizobium spp. The infected root system was analysed in the EMBRAPA/CNPH (National Research Centre for Vegetable Crops) Nematology Laboratory, Brasilia, Brazil.Research at Virginia Tech on the morphology and host range revealed several unusual morphological characters and a unique host range that indicated it was a new species. The perineal pattern, shape of the female stylet and shape of the male stylet were unique and different from those of any other described species. Meloidogyne
Bipolaris maydis was consistently isolated from infected Paspalum atratum cv. Pojuca plants showing leaf spot symptoms in the Cerrado of Brazil, in 2002. Pathogenicity tests under greenhouse conditions and subsequent reisolations of B. maydis from artificially inoculated Pojuca seedlings confirmed the hypothesis that this fungus was the causal agent of the disease. Symptoms of leaf spot appeared four days after inoculation in 100% of the inoculated Pojuca plants. All seven species of grasses evaluated were susceptible to B. maydis. The occurrence of leaf spot of Pojuca caused by B. maydis is reported for the first time in Brazil.
Colletotrichum acutatum foi consistentemente isolado de plantas de pequizeiro (Caryocar brasiliense) com sintomas de antracnose nas folhas, no Distrito Federal, em 2000. Testes de patogenicidade em casa de vegetação e o subsequente reisolamento do fungo confirmaram a hipótese de que C. acutatum é o agente etiológico da antracnose do pequizeiro. Os sintomas apareceram quatro dias após a inoculação nas 20 plantas inoculadas. Dezoito espécies vegetais testadas foram suscetíveis ao fungo. A antracnose do pequizeiro causada por C. acutatum é relatada pela primeira vez no Brasil
Meloidogyne phaseoli n. sp. is described and illustrated from specimens parasitising bean cv. Carioca in Brasilia, Brazil. The perineal pattern of the female is rounded to oval-shaped with a dorsal arch that is flattened to moderately high and squarish, sometimes with rounded shoulders. The striae are moderately spaced and often distinctly forked in the lateral field. The female stylet is 14-19 µm long and has broad, distinctly set-off knobs and several small, rounded projections on the shaft. The excretory pore opens 34-82 µm from the head near the level of metacorpus. Males are 999-2105 µm in length and have a high, wide head cap that slopes posteriorly. The labial disc and medial lips are partially fused to form an elongated lip structure. The robust stylet is 20-26 µm long and has wide knobs that are distinctly set-off from the shaft which is marked by several small, round projections. Mean second-stage juvenile length is 464 µm. The head region is not annulated and the large labial disc and crescent-shaped medial lips are fused to form a dumbbell-shaped head cap. The stylet is 10.5-12 µm long and has rounded, posteriorly sloping knobs. The slender tail, 46-64 µm long, has large irregular-sized annules in the posterior region and a slightly rounded tip. The hyaline tail terminus is long, 10.5-19.3 µm. The esterase isozyme is a unique E3 phenotype having one weak and two strong bands. Tomato, tobacco, bean and pea are good hosts, corn is a very poor host, whilst pepper, watermelon, peanut, cotton and soybean are non-hosts.Bean plants (Phaseolus vulgaris L. cv. Carioca) were collected from the commercial property of Fazenda Irméos Maldane, located in the area named 'Plano de Assentamento Dirigido' do DF (PADEF) in Brasilia, Brazil, with root systems highly infected with root-knot nematodes. The infected root systems were analysed at EM-BRAPA/CNPH, National Research Center for Vegetable Crops, Nematology Laboratory, Brasilia, Brazil, in September 1988. Symptoms expressed included chlorosis, stunting, root-rot and the total absence of Rhizobium nodulation.Additional research conducted at Virginia Tech, Department of Plant Pathology, Physiology and Weed Science, on the morphology, cytology, mode of reproduction and host-range, revealed several unusual features peculiar
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