Abstract. The regulation of human Factor IX.was studied in vitro in human and mouse plasma and in vivo in the mouse. In human plasma, -60% of the '251-Factor IX8 was bound to antithrombin III (ATIII) by 2 h, with no binding to a2-macroglobulin or aI-proteinase inhibitor, as assessed by gel electrophoresis and IgG-antiproteinase inhibitor-Sepharose beads. In the presence ofheparin, virtually 100% ofthe 125I-Factor IXa was bound to ATIII by 1 min.
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