In this study, biocontrol of harmful effect of cyanobacterial blooms and their toxins by “flocculation-biosorption” was achieved. Five fungal species were isolated from decayed cyanobacterial bloom which are: Aspergillus fumigatus, A. niger, Penicillium, Trichoderma ressei and Mucor rouxii. We chose the last species’ pellets because they are the most stable and cocultured with Anabeana sp. (1:5 fungal: cyanobacteria ratio) of dry weight, Harvest Efficacy HE% by fungal pellets started after 12h of co-culturing about (4%) and almost complete harvesting after 48h with (98%), then we add 0.1g of Magnetite nano Fe3o4 to facilitate removing cyanobacterial blooms. Microcystin-LR extracted from Anabaena sp. were purified and collected by preparative high-performance liquid chromatography (HPLC) was 75.1 (µg ml-1), M. rouxii pellet absorbed about 85% of Microcystin-LR after 72 h of incubation at 25 °C.
Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay.
Results:The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose.
Conclusion:This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.
The presents study included bioseparation and purification of hepatotoxins microcystin-LR from cyanobacteria species westeillopsis prolifica the concentration of toxin was determined by preparative high performance liquid chromatography by comparing peak area and retention time of analytical standard of microcystin-LR with peak area and retention time of extraction of each species of cyanobacteria, the retention time of analytical standard of microcystin-LR were 9.55 min and it’s concentration was 10µg/ml, W. prolifica retention time was 9.5 min, it’s concentration was 28.385 µg/ml.
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