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A pot experiment was conducted at Plant Breeding and Biotechnology Laboratory of Agrotechnology Discipline, Khulna University, during the period from Yellow Anne) were used as treatment variables. Layout system was Completely Randomized Design (CRD) under factorial arrangement with three replications. Spray formulation had significant influence on growth of the two cultivars tested. Significantly highest plant height was obtained (39.83 cm) at 290 DAP from Mokara Diehard Red Variety with spray formulation 3 compared to other spray formulations. Highest leaf area index (0.16) was obtained from Mokara Diehard Red Variety with spray formulation 3 at 290 DAP mainly due to increased number of leaf and total leaf area. Mokara Diehard Red cultivar with spray formulation 2 enhanced plant growths and resulted greater leaf number (21.33) and leaf length (26.83 cm) to other cultivars cultivated. Plant height as well as other growth parameters was gradually increased due to suitable treatment combination. Results of the study revealed that spray formulation 3 would be suitable for Mokara Diehard Red cultivars cultivation under pot condition.

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